利用原子力显微镜筛选 DNA 适体。
Selection of DNA aptamers using atomic force microscopy.
机构信息
Department of Chemical Science and Engineering Faculty of Engineering, Graduate School of Engineering, Kobe University, Rokkoudai-chou 1-1, Nada, Kobe 657-8501, Japan.
出版信息
Nucleic Acids Res. 2010 Mar;38(4):e21. doi: 10.1093/nar/gkp1101. Epub 2009 Dec 2.
Atomic force microscopy (AFM) can detect the adhesion or affinity force between a sample surface and cantilever, dynamically. This feature is useful as a method for the selection of aptamers that bind to their targets with very high affinity. Therefore, we propose the Systematic Evolution of Ligands by an EXponential enrichment (SELEX) method using AFM to obtain aptamers that have a strong affinity for target molecules. In this study, thrombin was chosen as the target molecule, and an 'AFM-SELEX' cycle was performed. As a result, selected cycles were completed with only three rounds, and many of the obtained aptamers had a higher affinity to thrombin than the conventional thrombin aptamer. Moreover, one type of obtained aptamer had a high affinity to thrombin as well as the anti-thrombin antibody. AFM-SELEX is, therefore, considered to be an available method for the selection of DNA aptamers that have a high affinity for their target molecules.
原子力显微镜（AFM）可以动态检测样品表面和悬臂之间的粘附力或亲和力。这一特性可用作一种方法来选择与靶标具有非常高亲和力的适体。因此，我们提出了一种使用 AFM 的系统进化配体的指数富集（SELEX）方法，以获得对靶分子具有强亲和力的适体。在这项研究中，选择凝血酶作为靶分子，并进行了“AFM-SELEX”循环。结果，仅用三轮就完成了选择循环，并且许多获得的适体对凝血酶的亲和力比传统的凝血酶适体高。此外，获得的一种适体对凝血酶和抗凝血酶抗体具有高亲和力。因此，AFM-SELEX 被认为是一种可用于选择对靶分子具有高亲和力的 DNA 适体的方法。
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