用于均相DNA传感的适体酶亚基。

Aptameric enzyme subunit for homogeneous DNA sensing.

作者信息

Ikebukuro Kazunori, Yoshida Wataru, Sode Koji

机构信息

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.

出版信息

Biotechnol Lett. 2008 Feb;30(2):243-52. doi: 10.1007/s10529-007-9526-z. Epub 2007 Sep 12.

DOI:10.1007/s10529-007-9526-z

PMID:17849085

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7087721/

Abstract

We have developed an aptameric enzyme subunit (AES) which can detect the DNA in a homogeneous solution. The AES is an artificial enzyme subunit composed of an enzyme-inhibiting aptamer bearing a target-molecule binding site. We connected a probe DNA to a thrombin-inhibiting aptamer at its 5' or 3' end. The inhibitory activity of the thrombin-inhibiting aptamer bearing the probe DNA decreased compared to that of the original aptamer; however, it recovered upon hybridization with the target DNA. Using this AES, we were able to detect target DNAs by measuring the thrombin activity in a homogeneous solution.

摘要

我们开发了一种适体酶亚基（AES），它可以在均相溶液中检测DNA。AES是一种人工酶亚基，由带有靶分子结合位点的酶抑制适体组成。我们将探针DNA连接到凝血酶抑制适体的5'或3'末端。与原始适体相比，带有探针DNA的凝血酶抑制适体的抑制活性降低；然而，与靶DNA杂交后它恢复了活性。使用这种AES，我们能够通过测量均相溶液中的凝血酶活性来检测靶DNA。