Department of General, Gastrointestinal and Thoracic Surgery, Evangelic Hospital Bielefeld, DE-33617 Bielefeld, Germany.
Pathobiology. 2009;76(6):277-85. doi: 10.1159/000245893. Epub 2009 Nov 30.
The following database integrates results from published proteomics studies in human non-small cell lung cancer (NSCLC), with a focus on squamous cell cancers (SCC) and adenocarcinomas (AC).
Only studies on NSCLC were analyzed. Results from 12 studies were available, 5 studies on SCC, 4 on AC, 1 on AC and SCC, and 2 on NSCLC without further distinction. Human cancer tissues, paired normal tissues, paired cancer patient's sera, normal sera or human tumor cell lines were analyzed. Three technologies were applied: two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), differential in-gel electrophoresis (DIGE) and serological proteome analysis (SERPA).
The total number of NSCLC patients was 306. Out of 261 differentially expressed proteins, 192 proteins (74%) were mentioned in a single study, 40 (15%) were mentioned by 2 different studies. 18 (7%) in 3 studies, 4 (2%) in 4 studies, 2 (1%) in 5 studies, and a single protein was listed in 6 studies.
Overlapping of protein expression is low between SCC and AC. However, a few proteins appear to be upregulated in SCC and AC: triosephosphate isomerase, protein disulfide isomerase and phosphoglycerate mutase (phosphoglycerate kinase 1). These three proteins might be key players in human lung cancer.
本数据库整合了已发表的人类非小细胞肺癌（NSCLC）蛋白质组学研究结果，重点关注鳞状细胞癌（SCC）和腺癌（AC）。
仅分析 NSCLC 相关研究。共有 12 项研究的结果可用，其中 5 项研究针对 SCC，4 项研究针对 AC，1 项研究同时针对 SCC 和 AC，还有 2 项研究针对 NSCLC 但未进一步细分。研究分析了人类癌症组织、配对正常组织、配对癌症患者血清、正常血清或人类肿瘤细胞系。应用了三种技术：二维聚丙烯酰胺凝胶电泳（2D-PAGE）、差异凝胶电泳（DIGE）和血清蛋白质组分析（SERPA）。
共有 306 例 NSCLC 患者。在 261 个差异表达蛋白中，192 个蛋白（74%）仅在一项研究中被提及，40 个蛋白（15%）被两项不同的研究提及。18 个蛋白（7%）被三项研究提及，4 个蛋白（2%）被四项研究提及，2 个蛋白（1%）被五项研究提及，6 项研究中各有一个蛋白被提及。
SCC 和 AC 之间的蛋白表达重叠较低。然而，有一些蛋白似乎在 SCC 和 AC 中上调：磷酸丙糖异构酶、蛋白二硫键异构酶和磷酸甘油酸变位酶（磷酸甘油酸激酶 1）。这三种蛋白可能是人类肺癌的关键参与者。
