Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
Int J Gynecol Cancer. 2009 Dec;19(9):1642-8. doi: 10.1111/IGC.0b013e3181a83555.
A study of human papilloma virus (HPV) types and variants is important for developing preventive protocols and appropriate intervention targets. The presence of HPV types, their variants, and viral load in a population subset from North India was studied. Polymerase chain reaction (PCR) and line blots were used for HPV genotyping; HPV 16 and 18 viral loads were measured using real-time PCR. Variant analysis was done by sequencing of the PCR-amplified E6/E7 regions of HPV 16 and the long control region and E6/E7 regions of HPV 18. The 93.6%, 78.6%, and 10% of tumors, squamous intraepithelial lesions (SILs), and controls were HPV-positive, respectively. The most commonly observed type was HPV 16. Human papilloma virus 73 which is uncommonly observed was seen in 2 tumors. Multiple infections were more common in controls and SILs than tumors. The majority (86.4%) of the HPV 16-positive and all of the HPV 18-positive samples belonged to the European variant class. Five novel nonsynonymous changes were seen in the HPV 16-positive and 2 in HPV 18-positive samples. There was a significant increase in viral loads from controls through SILs to tumors, but no significant differences in viral loads were observed between different stages of cancer. In tumors, a significant increase in HPV 16 viral loads was seen with increasing age. The study shows a similar HPV type and variant distribution to European studies, with some differences in type distribution. Viral load does not appear to be good marker for stage wise progression and intralesional variability may affect its use as a differentiating parameter between high-grade squamous intraepithelial lesion and low-grade squamous intraepithelial lesions.
研究人乳头瘤病毒（HPV）类型和变体对于制定预防方案和确定适当的干预靶点非常重要。本研究旨在探讨印度北部人群 HPV 类型、变体及其病毒载量的分布情况。采用聚合酶链反应（PCR）和线印迹法进行 HPV 基因分型；采用实时 PCR 法检测 HPV16 和 HPV18 的病毒载量。对 HPV16 的 E6/E7 区和长控制区以及 HPV18 的 E6/E7 区进行 PCR 扩增后进行序列分析，以检测 HPV 变体。结果显示，93.6%、78.6%和 10%的肿瘤、鳞状上皮内病变（SIL）和对照组织为 HPV 阳性，最常见的 HPV 类型为 HPV16。在 2 例肿瘤中观察到一种罕见的 HPV 类型 73。多重感染在对照组和 SIL 中比在肿瘤中更为常见。大多数（86.4%）HPV16 阳性和所有 HPV18 阳性样本均属于欧洲变体类型。在 HPV16 阳性样本中观察到 5 个新的非同义突变，在 HPV18 阳性样本中观察到 2 个。HPV 病毒载量从对照组经 SIL 到肿瘤呈显著升高，但在不同癌症阶段，HPV 病毒载量无显著差异。在肿瘤中，HPV16 病毒载量随年龄的增加而显著升高。本研究显示 HPV 类型和变体分布与欧洲研究相似，但 HPV 类型分布存在差异。病毒载量似乎不是评估疾病进展的良好标志物，肿瘤内的异质性可能影响其作为高级别鳞状上皮内病变和低级别鳞状上皮内病变的区分参数。
