那不勒斯硫杆菌中1,5-二磷酸核酮糖羧化酶/加氧酶的原位测定
In situ assay of ribulose-1,5-bisphosphate carboxylase/oxygenase in Thiobacillus neapolitanus.
作者信息
Cannon G C, English R S, Shively J M
机构信息
Department of Biological Sciences, Clemson University, South Carolina 29634.
出版信息
J Bacteriol. 1991 Feb;173(4):1565-8. doi: 10.1128/jb.173.4.1565-1568.1991.
Abstract
Cells permeabilized with chloroform yielded ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) activities nearly equal to those of cell extracts, thus indicating that both cytoplasmic and carboxysomal RuBisCO are functional in situ. The carboxysomal and cytoplasmic RuBisCO both form the CO2-Mg2(+)-enzyme ternary complex, as evidenced by stabilization with 2-C-carboxy-D-arabinitol-1,5-bisphosphate (CABP), a potent competitive inhibitor of RuBisCO. The data are consistent with the hypothesis that the carboxysome is functional in carbon dioxide fixation.
摘要
用氯仿透化处理的细胞产生的1,5-二磷酸核酮糖羧化酶/加氧酶（RuBisCO）活性几乎与细胞提取物的活性相当，这表明细胞质和羧基体中的RuBisCO在原位均具有功能。羧基体和细胞质中的RuBisCO都能形成CO₂-Mg²⁺-酶三元复合物，这一点通过用2-C-羧基-D-阿拉伯糖醇-1,5-二磷酸（CABP）（一种有效的RuBisCO竞争性抑制剂）进行稳定化处理得到了证明。这些数据与羧基体在二氧化碳固定中发挥作用的假设一致。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The thiobacilli.硫杆菌
Bacteriol Rev. 1957 Sep;21(3):195-213. doi: 10.1128/br.21.3.195-213.1957.
3
Interaction of ribulosebisphosphate carboxylase/oxygenase with transition-state analogues.核酮糖二磷酸羧化酶/加氧酶与过渡态类似物的相互作用。
Biochemistry. 1980 Mar 4;19(5):934-42. doi: 10.1021/bi00546a018.
4
Inclusion bodies of prokaryotes.原核生物的包涵体。
Annu Rev Microbiol. 1974;28(0):167-87. doi: 10.1146/annurev.mi.28.100174.001123.
5
Functional organelles in prokaryotes: polyhedral inclusions (carboxysomes) of Thiobacillus neapolitanus.原核生物中的功能性细胞器：那不勒斯硫杆菌的多面体内含物（羧酶体）
Science. 1973 Nov 9;182(4112):584-6. doi: 10.1126/science.182.4112.584.
6
Electron microscopy of the carboxysomes (polyhedral bodies) of Thiobacillus neapolitanus.那不勒斯硫杆菌羧酶体（多面体）的电子显微镜观察。
J Bacteriol. 1973 Dec;116(3):1405-11. doi: 10.1128/jb.116.3.1405-1411.1973.
7
Functional inclusions in prokaryotic cells.
Int Rev Cytol. 1988;113:35-100. doi: 10.1016/s0074-7696(08)60846-3.
8
Carboxysomes and ribulose bisphosphate carboxylase/oxygenase.羧酶体与核酮糖二磷酸羧化酶/加氧酶
Adv Microb Physiol. 1988;29:115-64. doi: 10.1016/s0065-2911(08)60347-1.
9
The 5'-flanking region of the gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is crucial for growth of the cyanobacterium Synechococcus sp. strain PCC 7942 at the level of CO2 in air.核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基编码基因的5'-侧翼区域对于聚球藻属蓝细菌PCC 7942菌株在空气中二氧化碳水平下的生长至关重要。
J Bacteriol. 1989 Nov;171(11):6069-76. doi: 10.1128/jb.171.11.6069-6076.1989.
10
Facile assay of enzymes unique to the Calvin cycle in intact cells, with special reference to ribulose 1,5-bisphosphate carboxylase.完整细胞中卡尔文循环特有的酶的简易测定，特别提及核酮糖1,5 - 二磷酸羧化酶。
Anal Biochem. 1978 Feb;84(2):462-72. doi: 10.1016/0003-2697(78)90064-7.
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