Mubeen Bismillah, Hasnain Ammarah, Mehboob Riffat, Rasool Rabia, Riaz Ayesha, Elaskary Shymaa Abdelsattar, Shah Muhammad Muntazir, Faridi Tallat Anwar, Ullah Inam
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan.
Lahore Medical Research Centre, LLP and LMRC Laboratories, Lahore, Pakistan.
Front Plant Sci. 2022 Aug 10;13:897795. doi: 10.3389/fpls.2022.897795. eCollection 2022.
Medicinal plants have been used to cure human diseases since decades. , a medicinal plant, is regarded as a source of secondary metabolites with therapeutic value against liver diseases and diabetes. The present study was conducted to enrich the production of secondary metabolites in the vegetative parts of using elicitation strategy in hydroponic system with different elicitors. The elicitors of fungus (0.2 g/L), methyl jasmonate (MeJA) (100 μM) and silver nanoparticles (AgNPs) (1 ppm) were added in hydroponic medium, individually and in combination form to the 15 days old plant. The elicitor-treated plants were harvested at different time points (24-144 h; increment 24 h) and their biochemical parameters like phenolics, flavonoids, nitric oxide (NO), and superoxide dismutase (SOD) were analyzed. The results showed hyper-accumulation of these biochemical contents, especially in response to MeJA (100 μM), followed by AgNPs (1 ppm) and co-treatment of AgNPs (1 ppm) with other elicitors. The results revealed that the treatment with MeJA (100 μM) exhibited the highest flavonoid (304 μg g), phenolic (372 μg g), and SOD (16.2 U g) contents. For NO levels, the maximum value of 198.6 nmole g was achieved in response to the treatment with MeJA + Green synthesized AgNPs (100 μM + 1 ppm). Our findings depicted an enhanced production of medicinally important plant secondary metabolites and antioxidants; hence, the method applied in this study can play a significant role to improve therapeutic values of the plants.
几十年来，药用植物一直被用于治疗人类疾病。[植物名称]是一种药用植物，被认为是具有治疗肝病和糖尿病价值的次生代谢产物的来源。本研究旨在通过在水培系统中使用不同诱导剂的诱导策略，提高[植物名称]营养器官中次生代谢产物的产量。将真菌诱导剂（0.2 g/L）、茉莉酸甲酯（MeJA）（100 μM）和银纳米颗粒（AgNPs）（1 ppm）分别或组合添加到水培培养基中，处理15日龄的植株。在不同时间点（24 - 144小时；间隔24小时）收获经诱导剂处理的植株，并分析其酚类、黄酮类、一氧化氮（NO）和超氧化物歧化酶（SOD）等生化参数。结果表明，这些生化成分有超积累现象，尤其是对MeJA（100 μM）的响应，其次是AgNPs（1 ppm）以及AgNPs（1 ppm）与其他诱导剂的共同处理。结果显示，用MeJA（100 μM）处理的植株黄酮类（304 μg/g）、酚类（372 μg/g）和SOD（16.2 U/g）含量最高。对于NO水平，在用MeJA + 绿色合成的AgNPs（100 μM + 1 ppm）处理时达到最大值198.6 nmole/g。我们的研究结果表明药用植物次生代谢产物和抗氧化剂的产量有所提高；因此，本研究中应用的方法对于提高植物的治疗价值可发挥重要作用。
