Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr University Bochum, Bochum, Germany.
Arch Dermatol Res. 2010 Jul;302(5):357-65. doi: 10.1007/s00403-009-1009-8. Epub 2009 Dec 3.
Wound infections with multi-drug resistant bacteria increase morbidity and mortality and have considerable socioeconomic impact. They can lead to impaired wound healing, resulting in rising treatment costs. The aim of this study was to investigate an ex vivo human wound infection model. Human full-thickness skin from the operating room (OR) was placed into the Bo-Drum and cultivated for 7 days in an air-liquid interphase. On day 8, the skin was inoculated with either (1) Pseudomonas aeruginosa, (2) Staphylococcus aureus (10(5) CFU, n = 3) or (3) carrier control. 1, 3 and 7 days after inoculation colony forming units in the tissue/media were determined and cytokine expression was quantified. A reliable and reproducible wound infection could be established for 7 days. At this time point, 1.8 x 10(8) CFU/g tissue of P. aeruginosa and 2 x 10(7) CFU/g tissue of S. aureus were detected. Immunohistochemical analysis demonstrated bacterial infection and epidermolysis in infected skin. RT-PCR analysis exhibited a significant induction of proinflammatory cytokines after infection. The BO-drum is a robust, easy-to-use, sterilizable and reusable ex vivo full-skin culture system. For investigation of wound infection, treatment and healing, the BO-drum presents a convenient model and may help to standardize wound research.
耐药菌引起的伤口感染会增加发病率和死亡率，并对社会经济产生重大影响。它们会导致伤口愈合受损，从而增加治疗成本。本研究旨在调查一种体外人类伤口感染模型。从手术室（OR）获取的全厚皮肤被放置在 Bo-Drum 中，并在气液界面中培养 7 天。在第 8 天，皮肤接种了（1）铜绿假单胞菌、（2）金黄色葡萄球菌（10(5) CFU，n = 3）或（3）载体对照。接种后 1、3 和 7 天，测定组织/培养基中的菌落形成单位，并定量细胞因子表达。可以建立可靠且可重复的 7 天伤口感染模型。此时，检测到 P. aeruginosa 的 1.8 x 10(8) CFU/g 组织和 S. aureus 的 2 x 10(7) CFU/g 组织。免疫组织化学分析显示感染皮肤存在细菌感染和表皮松解。RT-PCR 分析显示感染后促炎细胞因子的显著诱导。BO-drum 是一种强大、易于使用、可消毒和可重复使用的体外全皮肤培养系统。对于伤口感染、治疗和愈合的研究，BO-drum 提供了一种便捷的模型，并有助于规范伤口研究。
