School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
Phytother Res. 2010 Jun;24 Suppl 2:S183-9. doi: 10.1002/ptr.3068.
The study was designed to evaluate the effects of 1 microM beta-carotene on antioxidant status in ethanol-treated rat hepatocytes and investigate possible anti-apoptotic mechanisms of beta-carotene in protecting ethanol-induced cytotoxicity. The isolated rat hepatocytes were incubated for 48 h in a medium with or without alcohol (100 mM) and mu-carotene (1 microM) using the following groups: the control (C), beta-carotene (CB), ethanol (E), and ethanol + beta-carotene (EB) groups. The cell viability, antioxidative status, cytochrome P450 2E1 (CYP2E1) and caspase expressions in hepatocytes were measured. The E group demonstrated lower cell viability, glutathione (GSH) levels, and lipid peroxide accumulation in rat hepatocytes; meanwhile, CYP2E1, caspase-3, and caspase-9 expressions increased. In contrast, cell viability, GSH levels, and glutathione reductase (GRD) activity significantly increased while lipid peroxides and expressions of CYP2E1, casapse-3, and caspase-9 decreased in the EB group. The results suggest that ethanol treatment decreases cell viability in rat hepatocytes via induced oxidative stress. 1 muM beta-carotene decreased oxidative stress and prevented ethanol-induced cell death by inhibiting caspase-9 and caspase-3 expression.
这项研究旨在评估 1 微摩尔β-胡萝卜素对乙醇处理的大鼠肝细胞抗氧化状态的影响，并研究β-胡萝卜素在保护乙醇诱导的细胞毒性中的可能抗凋亡机制。将分离的大鼠肝细胞在含有或不含有酒精（100mM）和β-胡萝卜素（1 微摩尔）的培养基中孵育 48 小时，使用以下组：对照组（C）、β-胡萝卜素组（CB）、乙醇组（E）和乙醇+β-胡萝卜素组（EB）。测量肝细胞的细胞活力、抗氧化状态、细胞色素 P450 2E1（CYP2E1）和半胱氨酸蛋白酶的表达。E 组显示大鼠肝细胞的细胞活力、谷胱甘肽（GSH）水平和脂质过氧化物积累降低；同时，CYP2E1、半胱氨酸蛋白酶-3 和半胱氨酸蛋白酶-9 的表达增加。相比之下，在 EB 组中，细胞活力、GSH 水平和谷胱甘肽还原酶（GRD）活性显著增加，而脂质过氧化物和 CYP2E1、半胱氨酸蛋白酶-3 和半胱氨酸蛋白酶-9 的表达减少。结果表明，乙醇处理通过诱导氧化应激降低大鼠肝细胞的细胞活力。1 微摩尔β-胡萝卜素通过抑制半胱氨酸蛋白酶-9 和半胱氨酸蛋白酶-3 的表达来减少氧化应激并防止乙醇诱导的细胞死亡。
