Université de Lyon, France.
Anat Rec (Hoboken). 2010 Feb;293(2):215-24. doi: 10.1002/ar.21054.
The objective of this study was to develop and validate a technique for both 3D imaging and quantification of the vascular network of bone tissue in the rat. Five month-old male Wistar rats were divided into tail-suspension (21 days) and control groups. Sixty percent barium sulfate solution was infused into the vena cava. The tibiae were evaluated in 2D and 3D before and after decalcification, using conventional microcomputerized tomography (muCT) at 10 and 5 mum resolution and synchrotron radiation (SR) muCT. The perfusion technique and tomography exhibited excellent bone vasculature imaging. Significant positive correlations were observed between 2D histomorphometric and 3D muCT vascular parameters (P < 0.05). 3DmuCT discriminated significant changes of vessel structures in unloading condition: vessel number decreased by 25%, (P < 0.005), vessel separation increased by 27%, P < 0.01. SRmuCT could image sinusoid clusters in bone. muCT is an accurate and reproducible technique for 3D quantitative evaluation of long bone vascularisation in the rat.
本研究旨在开发和验证一种用于大鼠骨组织血管网络的 3D 成像和定量分析的技术。将 5 月龄雄性 Wistar 大鼠分为尾吊（21 天）组和对照组。60%硫酸钡溶液注入腔静脉。在脱钙前后，使用传统的微机化断层扫描（μCT）以 10 和 5μm 分辨率和同步辐射（SR）μCT 进行二维和三维评估。灌注技术和断层扫描显示出出色的骨血管成像效果。二维组织形态计量学和 3DμCT 血管参数之间存在显著的正相关关系（P<0.05）。3DμCT 区分了卸载条件下血管结构的显著变化：血管数量减少了 25%（P<0.005），血管分离增加了 27%，P<0.01。SRμCT 可以在骨中成像窦状cluster。μCT 是一种准确且可重复的技术，可用于大鼠长骨血管化的 3D 定量评估。
