The Medical School, Ningbo University, Ningbo, China 315211.
J Biomed Mater Res B Appl Biomater. 2010 Feb;92(2):508-16. doi: 10.1002/jbm.b.31544.
Design of polymeric scaffolds with specific physical and biological properties is a key objective of tissue engineering research. Electrospinning generates loosely connected 3D porous mats simulating extra cellular matrix structure and therefore makes itself an excellent candidate for application in tissue engineering. Besides a high voltage generator and syringe pump, our electrospinning system was improved to add a programmable central controller which monitors system operation. The nozzles connected with syringe pump via silicon rubber tubing can move linearly with a step size of 0.1 microm or above while the mandrel collector rotates at a speed from 400 to 3000 revolutions per minute (rpm). Using this system, porous fiber sheets with fiber diameters ranging from 100 nm to several micrometers or meshes of macroscopically aligned fibers with diameter of approximately 10 microm have been fabricated under proper processing conditions. After biocompatible fibrin coating, oriented polycaprolactone (PCL) fibers were found to enhance the shifting of human umbilical artery smooth muscle cells from synthetic to contractile phenotype, and to maintain biological function of human umbilical vein endothelial cells. We believe that our electrospinning system will facilitate scaffold fabrication for vessel tissue engineering.
设计具有特定物理和生物学特性的聚合物支架是组织工程研究的一个关键目标。静电纺丝产生松散连接的 3D 多孔垫，模拟细胞外基质结构，因此成为组织工程应用的优秀候选者。除了高压发生器和注射器泵之外，我们的静电纺丝系统经过改进，增加了一个可编程中央控制器，可监控系统运行。通过硅橡胶管与注射器泵连接的喷嘴可以以 0.1 微米或以上的步长线性移动，而芯轴收集器以每分钟 400 到 3000 转的速度旋转。在适当的处理条件下，使用该系统可以制造出纤维直径从 100nm 到几微米的多孔纤维片，或者直径约为 10μm 的宏观排列纤维的网。经过生物相容性纤维蛋白涂层后，定向聚己内酯（PCL）纤维被发现可增强人脐动脉平滑肌细胞从合成到收缩表型的迁移，并维持人脐静脉内皮细胞的生物学功能。我们相信我们的静电纺丝系统将有助于血管组织工程支架的制造。
