Department of Biochemistry, University of Allahabad, Allahabad 211002, India.
Z Naturforsch C J Biosci. 2009 Sep-Oct;64(9-10):626-30. doi: 10.1515/znc-2009-9-1004.
Oxidative stress is believed to be a major contributing factor in the development of late complications of diabetes. Many in vitro and in vivo studies have demonstrated that several parameters of red blood cell function and integrity are negatively affected by increased oxidative stress. Plant polyphenols are reported to exert many biological effects due to their antioxidant property. The present study was undertaken to evaluate the antioxidant effect of myricetin on markers of oxidative stress in erythrocytes from type 2 diabetic patients. The study was carried out on blood samples obtained from 23 type 2 diabetic patients and 23 age-matched control subjects. Erythrocytes were subjected to in vitro oxidative stress by incubating with 10(-5) M tert-butyl hydroperoxide (t-BHP). Erythrocyte membrane lipid peroxidation and protein oxidation were measured in terms of malondialdehyde (MDA) and protein carbonyl group levels. The results showed an elevated MDA and protein carbonyl content in diabetic erythrocytes which were further increased after incubation with t-BHP. Myricetin at micromolar concentration significantly (p < 0.01) protected an t-BHP-induced increase in levels of oxidative stress parameters of diabetic erythrocytes.
氧化应激被认为是糖尿病晚期并发症发展的一个主要因素。许多体外和体内研究表明，红细胞功能和完整性的几个参数受到氧化应激增加的负面影响。植物多酚由于其抗氧化特性而具有许多生物学作用。本研究旨在评估杨梅素对 2 型糖尿病患者红细胞氧化应激标志物的抗氧化作用。该研究在 23 名 2 型糖尿病患者和 23 名年龄匹配的对照组的血液样本上进行。通过用 10(-5) M 叔丁基过氧化物 (t-BHP) 孵育，使红细胞产生体外氧化应激。用丙二醛 (MDA) 和蛋白质羰基含量来衡量红细胞膜脂质过氧化和蛋白质氧化。结果表明，糖尿病患者的红细胞 MDA 和蛋白质羰基含量升高，用 t-BHP 孵育后进一步增加。在微摩尔浓度下，杨梅素显著 (p < 0.01) 保护糖尿病红细胞中 t-BHP 诱导的氧化应激参数的增加。
