Laboratory of Nutrition, Department of Science of Food Function and Health, Graduate School of Agricultural Science, Tohoku University 1-1 Tsutsumidori-Amamiyamachi, Sendai 981-8555, Japan.
J Agric Food Chem. 2010 Jan 27;58(2):835-41. doi: 10.1021/jf9031487.
Collagen, a major extracellular matrix macromolecule, is widely used for biomedical purposes. We investigated the absorption mechanism of low molecular weight collagen hydrolysate (LMW-CH) and its effects on osteoporosis in rats. When administered to Wistar rats with either [(14)C]proline (Pro group) or glycyl-[(14)C]prolyl-hydroxyproline (CTp group), LMW-CH rapidly increased plasma radioactivity. LMW-CH was absorbed into the blood of Wistar rats in the peptide form. Glycyl-prolyl-hydroxyproline tripeptide remained in the plasma and accumulated in the kidney. In both groups, radioactivity was retained at a high level in the skin until 14 days after administration. Additionally, the administration of LMW-CH to ovariectomized stroke-prone spontaneously hypertensive rats increased the organic substance content and decreased the water content of the left femur. Our findings show that LMW-CH exerts a beneficial effect on osteoporosis by increasing the organic substance content of bone.
胶原蛋白是一种主要的细胞外基质大分子，广泛用于生物医学目的。我们研究了低分子量胶原蛋白水解物（LMW-CH）的吸收机制及其对大鼠骨质疏松症的影响。当给用 [(14)C]脯氨酸（Pro 组）或甘氨酰-[(14)C]脯氨酰-羟脯氨酸（CTp 组）标记的 Wistar 大鼠施用 LMW-CH 时，LMW-CH 迅速增加血浆放射性。LMW-CH 以肽的形式被吸收到 Wistar 大鼠的血液中。甘氨酰-脯氨酰-羟脯氨酸三肽仍留在血浆中并在肾脏中积累。在这两组中，放射性在给药后 14 天内仍保持在皮肤中的高水平。此外，给卵巢切除的易发生中风的自发性高血压大鼠施用 LMW-CH 增加了左股骨的有机物质含量并降低了水含量。我们的研究结果表明，LMW-CH 通过增加骨的有机物质含量对骨质疏松症发挥有益作用。
