Chair for Analytical Chemistry, Technische Universität München, Marchioninistrasse 17, D 81377 Munich, Germany.
Anal Chem. 2010 Jan 1;82(1):441-6. doi: 10.1021/ac902573p.
We present a rapid and straightforward procedure for the synthesis of core-shell surface-enhanced Raman scattering (SERS) tags. SERS tags are combinations of Raman active dyes with metallic nanoparticles inducing the enhancement. Because of their significantly higher stability to environmental conditions, the majority of SERS tags are designed in core-shell geometry. The main disadvantage of this approach up to now was the time-consuming (normally days) and complex preparation of these labeled core-shell particles. The presented approach can be performed within hours on a daily base. The main characteristics and the stability of the fabricated SERS tags in extreme pH values, high ion strength, or in organic solvent is demonstrated in this communication. Furthermore, the SERS tags are functionalized with anti-Salmonella antibody as a model to present a potential application of the core-shell particles in bioimaging.
我们提出了一种快速而直接的方法来合成核壳型表面增强拉曼散射（SERS）标记物。SERS 标记物是将拉曼活性染料与诱导增强的金属纳米粒子结合而成。由于它们对环境条件具有更高的稳定性，大多数 SERS 标记物都设计为核壳结构。到目前为止，这种方法的主要缺点是制备这些标记核壳颗粒既耗时（通常需要数天）又复杂。本研究提出的方法可以在每天的基础上在数小时内完成。本文介绍了所制备的 SERS 标记物在极端 pH 值、高离子强度或有机溶剂中的主要特性和稳定性，并以抗沙门氏菌抗体功能化为模型，展示了核壳粒子在生物成像中的潜在应用。
