Graduate School of Engineering, Nagoya University, Nagoya, Japan.
Tissue Eng Part C Methods. 2010 Oct;16(5):947-56. doi: 10.1089/ten.TEC.2009.0305.
Despite the growing number of clinically practical automated cell culture systems, demand is also increasing for more compact platforms with greater capabilities to prepare primary cells directly from patient tissue. Here we report the development of an automated cell culture system that is also compact. The machinery consisted of a supply unit, an incubation unit, and a collection unit, which fit within a 70 cm x 60 cm x 86 cm space. The compact size was enabled by our concept of using a single culture vessel from the primary culture steps to final cell harvest instead of scaling up with multiple culture vessels. Human fibroblasts and bone marrow stromal cells (BMSCs) were successfully cultured with this system over 19 days without contamination. From three pieces of gingival tissue (2 mm x 2 mm) or from 10 mL of bone marrow aspirate, the system could produce more than 2.0x10(7) cells and up to 3.0x10(7) cells for fibroblasts and BMSCs, respectively. The BMSCs produced by this system were capable of ectopic bone formation after transplantation into the subcutaneous space of nude mice. Our prototype system will provide a foundation for minimizing automatic culture machinery with clinically relevant cell yields while also expanding the automation capabilities to include primary tissue culture.
尽管临床上实用的自动化细胞培养系统的数量不断增加，但人们也越来越需要更紧凑、功能更强大的平台，以便直接从患者组织中制备原代细胞。在这里，我们报告了一种自动化细胞培养系统的开发，该系统也很紧凑。该设备由供应单元、孵育单元和收集单元组成，可在 70 cm x 60 cm x 86 cm 的空间内使用。紧凑的尺寸得益于我们的理念，即用单个培养容器从原代培养步骤到最终细胞收获，而不是通过多个培养容器进行放大。该系统可成功培养人成纤维细胞和骨髓基质细胞（BMSCs），培养时间超过 19 天而无污染。从三块牙龈组织（2 mm x 2 mm）或 10 毫升骨髓抽吸物中，该系统可分别产生超过 2.0x10(7)个细胞和多达 3.0x10(7)个成纤维细胞和 BMSCs。该系统产生的 BMSCs 可在裸鼠皮下移植后异位形成骨。我们的原型系统将为最小化具有临床相关细胞产量的自动培养设备提供基础，同时还将自动化功能扩展到包括原代组织培养。
