Graduate School of Life and Environmental Sciences, Initiative for the Promotion of Young Scientists' independent Research, University of Tsukuba, Gene Research Center 220, 1-1-1 Ten-nou dai, Tsukuba 305-8572, Japan.
J Plant Physiol. 2010 May 1;167(7):555-60. doi: 10.1016/j.jplph.2009.11.003. Epub 2009 Dec 2.
Low temperature induces several genes to acquire plant cold tolerance. Here, we demonstrate that accumulation of salicylic acid (SA) is involved in the regulation of the DREB1A/CBF3 regulon and plant tolerance to cold stresses. The SA-accumulating mutant siz1 exhibits sensitivity to chilling and freezing conditions and decreased expression of DREB1A/CBF3 and its regulon genes. Reduction of SA levels in siz1 by nahG restored cold sensitivity and down-regulation of these genes. Database analyses and RT-PCR analysis revealed that the ice1 mutation also increased expression of SA-responsive genes. As well as siz1, another SA-accumulating mutant acd6 exhibited freezing sensitivity and the sensitivity was suppressed in acd6 nahG plants. Taken together, these data indicate that SA is involved in regulation of cold signaling.
低温诱导几个基因获得植物耐寒性。在这里，我们证明水杨酸（SA）的积累参与了 DREB1A/CBF3 调控因子和植物对冷胁迫的耐受性的调节。积累 SA 的 siz1 突变体对冷胁迫和冻融条件敏感，并且 DREB1A/CBF3 及其调控因子基因的表达降低。通过 nahG 在 siz1 中降低 SA 水平恢复了冷敏感性和这些基因的下调。数据库分析和 RT-PCR 分析表明，ice1 突变也增加了 SA 应答基因的表达。与 siz1 一样，另一个积累 SA 的突变体 acd6 对冻融敏感，而在 acd6 nahG 植物中这种敏感性受到抑制。综上所述，这些数据表明 SA 参与了低温信号的调节。
