TSUJIO Masashi, MIZOROGI Toshihiro, KITAMURA Itsuko, MAEDA Yasuhiro, NISHIJIMA Kazutoshi, KUWAHARA Sachi, OHNO Tamio, NIIDA Shunpei, NAGAYA Masahiro, Saito Ryoichi, Tanaka Shin
Animal Facility for Aging Research (AFAR), National Center for Geriatrics and Gerontology (NCGG), Obu, Aichi, Japan.
J Vet Med Sci. 2009 Nov;71(11):1493-7. doi: 10.1292/jvms.001493.
To determine how to eliminate species difference in animal bone experiment, bone mineral content (BMC) was measured using dual energy X-ray absorptiometry (DXA) on the femurs of laboratory mice (Mus musculus) and rats (Rattus norvegicus), and common marmosets (Callithrix jacchus). Measures were taken on femurs in situ, detached from the body, skinned and defleshed, or dried completely. When the BMC of the bone measured in the intact limb attached to the trunk was set at 100%, the actual BMC of the dry bone was 58.7 +/- 11.5% in mice and 103.2 +/- 3.2% in rats. Similarly, the bone area (Area) and bone mineral density (BMD) of the dried femur was significantly lower in the mouse femurs than intact limb. Thus, soft limb tissue such as skin and muscle modified the BMC, Area, and BMD only in mouse but not in those from rats or marmosets. The bone mineral ratio (BMR; BMC divided by dry bone weight) was nearest to the human bone value in the rat femurs, whereas the mouse femur BMR was the most different. The BMR was proved to be a practical index in evaluating bone characteristics in laboratory animals, but the mouse femur might not be suitable as an animal model for research into the aging of human bone.
为了确定如何消除动物骨骼实验中的物种差异，我们使用双能X射线吸收法（DXA）测量了实验室小鼠（小家鼠）、大鼠（褐家鼠）和普通狨猴（狨猴）股骨的骨矿物质含量（BMC）。测量分别在原位股骨、离体股骨（去皮去肉）或完全干燥的股骨上进行。当将附着于躯干的完整肢体中测量的骨骼BMC设定为100%时，小鼠干燥骨骼的实际BMC为58.7±11.5%，大鼠为103.2±3.2%。同样，干燥股骨的骨面积（Area）和骨矿物质密度（BMD）在小鼠股骨中显著低于完整肢体。因此，皮肤和肌肉等柔软的肢体组织仅对小鼠的BMC、Area和BMD有影响，而对大鼠或狨猴的这些指标没有影响。大鼠股骨的骨矿物质比率（BMR；BMC除以干燥骨骼重量）最接近人类骨骼值，而小鼠股骨的BMR差异最大。BMR被证明是评估实验动物骨骼特征的一个实用指标，但小鼠股骨可能不太适合作为研究人类骨骼老化的动物模型。
