Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 710032, Xi'an, Shaanxi Province, China.
Dig Dis Sci. 2010 Sep;55(9):2545-51. doi: 10.1007/s10620-009-1051-6. Epub 2009 Dec 4.
So far, the miRNAs involved in multidrug resistance of esophageal cancer have not been reported.
Here we have firstly investigated the roles of miR-27a in multidrug resistance of esophageal squamous cell carcinoma using MTT assay, flow cytometry assay, and reporter gene assay, etc.
Down-regulation of miR-27a could confer sensitivity of both P-glycoprotein-related and P-glycoprotein-non-related drugs on esophageal cancer cells, and might promote ADR-induced apoptosis, accompanied by increased accumulation and decreased releasing amount of ADR. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein, Bcl-2, and the transcription of the multidrug resistance gene 1, but up-regulate the expression of Bax.
MiR-27a might play important roles in multidrug resistance of esophageal cancer. The further study of the biological functions of miR-27a might be helpful for developing possible strategies to treat esophageal cancer.
目前，涉及食管癌多药耐药的 miRNA 尚未见报道。
本研究采用 MTT 法、流式细胞术和报告基因等方法，首次探讨了 miR-27a 在食管鳞癌细胞多药耐药中的作用。
下调 miR-27a 可增加 P-糖蛋白相关和非相关药物对食管癌细胞的敏感性，并可能促进 ADR 诱导的细胞凋亡，同时增加 ADR 的蓄积和减少释放。下调 miR-27a 可显著降低 P-糖蛋白、Bcl-2 的表达和多药耐药基因 1 的转录，同时上调 Bax 的表达。
miR-27a 可能在食管癌多药耐药中发挥重要作用。对 miR-27a 生物学功能的进一步研究可能有助于开发治疗食管癌的可能策略。
