Zhou Yi, Hong Liu
Department of Colorectal Surgery, Tianjin Union Medicine Center, Tianjin, China.
Genet Test Mol Biomarkers. 2013 Jun;17(6):470-4. doi: 10.1089/gtmb.2012.0518. Epub 2013 May 13.
Here, we have investigated the role of miR-483 and miR-214 in the prognosis and multidrug resistance (MDR) of esophageal squamous cell carcinoma.
The expression of miR-483 and miR-214 was detected in 104 cases of esophageal cancer tissues and matched adjacent benign esophageal tissues by quantitative real-time PCR. The relation of microRNA expression with survival was statistically analyzed. The roles of miR-483 and miR-214 in MDR of esophageal squamous cell cancer cells were further evaluated.
The expression of miR-483 and miR-214 was found significantly upregulated in esophageal squamous cell cancer tissues. The expression levels of miR-483 and miR-214 showed an inverse correlation with overall survival. High expression of miR-483 and miR-214 might predict less chemotherapy effect. Downregulation of miR-483 and miR-214 could confer sensitivity of both P-glycoprotein-related and P-glycoprotein-nonrelated drugs to esophageal cancer cells, and it might induce increased accumulation of adriamycin (ADR) and decreased amount of ADR released.
miR-483 and miR-214 might play important roles in the pathogenesis of esophageal cancer and should be considered as potential targets for intervention in this malignancy.
在此，我们研究了miR - 483和miR - 214在食管鳞状细胞癌预后及多药耐药（MDR）中的作用。
采用定量实时聚合酶链反应（PCR）检测104例食管癌组织及配对的癌旁良性食管组织中miR - 483和miR - 214的表达。对微小RNA表达与生存的关系进行统计学分析。进一步评估miR - 483和miR - 214在食管鳞状细胞癌细胞多药耐药中的作用。
发现miR - 483和miR - 214在食管鳞状细胞癌组织中显著上调。miR - 483和miR - 214的表达水平与总生存期呈负相关。miR - 483和miR - 214的高表达可能预示化疗效果较差。下调miR - 483和miR - 214可使食管癌细胞对P - 糖蛋白相关和P - 糖蛋白非相关药物均产生敏感性，且可能导致阿霉素（ADR）蓄积增加及释放量减少。
miR - 483和miR - 214可能在食管癌发病机制中起重要作用，应被视为该恶性肿瘤干预的潜在靶点。
