Xiong Guang-Zong, Ye Kai-He, Huang Juan-Li, Ye Chun-Ling
Dept. Pharmacology, Pharmacy College of Jinan University, Guangzhou 510632, China.
Zhong Yao Cai. 2009 Aug;32(8):1238-41.
To investigate the changes of apelin in plasma and myocardium of model rats and the protective mechanisms of Extracts of Ginkgo biloba leaves (EGb) on myocardial ischemia injury induced by isoproterenol.
The model of myocardial ischemia injury was induced by subcutaneous injection of high dose isoproterenol. ELISA (enzyme linked immunosorbent assay) was used to measure the apelin concentration in plasma and myocardium. Semi-Quantitative RT-PCR was used to measure the apelin mRNA level in myocardium. The pathomorphology changes of myocardium was observed with light microscope. EGb was administered for 7 weeks.
Compared with the normal control group, the apelin concentration in plasma and myocardium and the apelin mRNA level in myocardium significantly decreased in the model group (P < 0.01). Compared with the model group, the apelin concentration in plasma and myocardium and the apelin mRNA level in myocardium obviously increased in the EGb group (P < 0.01). Meanwhile, the NO content in serum also obviously increased and the pathological damage of myocardium was obviously improved.
The protective mechanisms of EGb on myocardial ischemia injury may be related to the elevation of apelin contents and apelin mRNA level.
探讨模型大鼠血浆及心肌中apelin的变化以及银杏叶提取物（EGb）对异丙肾上腺素所致心肌缺血损伤的保护机制。
通过皮下注射高剂量异丙肾上腺素诱导心肌缺血损伤模型。采用酶联免疫吸附测定法（ELISA）检测血浆及心肌中apelin浓度。采用半定量逆转录聚合酶链反应（RT-PCR）检测心肌中apelin mRNA水平。用光镜观察心肌的病理形态学变化。EGb给药7周。
与正常对照组相比，模型组血浆及心肌中apelin浓度及心肌中apelin mRNA水平显著降低（P<0.01）。与模型组相比，EGb组血浆及心肌中apelin浓度及心肌中apelin mRNA水平明显升高（P<0.01）。同时，血清中一氧化氮（NO）含量也明显升高，心肌病理损伤明显改善。
EGb对心肌缺血损伤的保护机制可能与apelin含量及apelin mRNA水平升高有关。
