Molecular Biomedicine Unit, Research and Development Department, Institute of Immunology Inc, Rockefellerova 10, 10000 Zagreb, Croatia.
Vaccine. 2010 Feb 17;28(7):1887-92. doi: 10.1016/j.vaccine.2009.11.049. Epub 2009 Dec 2.
The two most commonly used methods for the determination of a virus potency are plaque assay and 50% cell culture infective dose (CCID(50)) assay, both based on cytopathic effect observation. We compared the potency estimates obtained by plaque and CCID(50) assays for nine mumps virus strains that produce different cytopathic effects in Vero cells. The ratios of CCID(50) and plaque assay quantification results differed for different strains and were in a range of 0.66-10, indicating that quantification results for some mumps virus strains are almost identical regardless of whether CCID(50) or plaque method is used, while the potency estimates of other strains strongly depend on the choice of the assay.
两种最常用于测定病毒效价的方法是噬斑测定法和 50%细胞培养感染剂量（CCID(50)）测定法，这两种方法均基于细胞病变效应观察。我们比较了在 Vero 细胞中产生不同细胞病变效应的九株腮腺炎病毒株的噬斑测定法和 CCID(50)测定法得到的效价估计值。不同毒株的 CCID(50)和噬斑测定法定量结果比值在 0.66-10 之间，这表明，无论使用 CCID(50)法还是噬斑法，一些腮腺炎病毒株的定量结果几乎完全相同，而其他毒株的效价估计值则强烈依赖于测定方法的选择。
