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利用固氮菌群落进行三氯乙烯的生物过滤。

Bio-filtration of trichloroethylene using diazotrophic bacterial community.

机构信息

Department of Botany, Banaras Hindu University, Varanasi 221005, India.

出版信息

Bioresour Technol. 2010 Apr;101(7):2126-33. doi: 10.1016/j.biortech.2009.10.094. Epub 2009 Dec 3.

DOI:10.1016/j.biortech.2009.10.094
PMID:19962302
Abstract

Biodegradation of TCE was studied in a biofilter packed with wood charcoal and inoculated with diazotrophic bacterial community isolated from local soil. Steady state TCE removal efficiencies higher than 85% were observed up to inlet load of 2.866 g m(-3) h(-1). The maximum elimination capacity of 5.31 g m(-3) h(-1) was observed at an inlet load of more than 7.90 g m(-3) h(-1). The biofilter was sensitive to fluctuations in the process conditions but could easily recover its performance after 10 days shutdown. Almost constant and small pressure drop per unit length and very negligible compaction was observed during the whole experimental period. The molecular analyses such as RT-PCR and gene sequencing revealed the presence of functionally active Azospirillum species in the biofilm.

摘要

采用从当地土壤中分离的固氮细菌群落接种的木炭填充生物滤池研究了三氯乙烯的生物降解。在入口负荷为 2.866 g m(-3) h(-1)时,观察到稳定状态的 TCE 去除效率高于 85%。在入口负荷大于 7.90 g m(-3) h(-1)时,观察到最大消除能力为 5.31 g m(-3) h(-1)。生物滤池对工艺条件的波动敏感,但在关闭 10 天后可轻松恢复性能。在整个实验期间,观察到单位长度的几乎恒定且较小的压降和非常可忽略不计的压实。RT-PCR 和基因测序等分子分析表明,生物膜中存在功能活跃的固氮螺旋菌属。

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