Department of Botany, Banaras Hindu University, Varanasi, India.
Bioresour Technol. 2011 Feb;102(3):3561-3. doi: 10.1016/j.biortech.2010.09.037. Epub 2010 Sep 17.
The kinetics of biodegradation of TCE in the biofilter packed with wood charcoal and inoculated with diazotrophic bacterial community had been investigated. Use of Michaelis-Menten type model showed that substrate inhibition was present in the system. The kinetic model proposed by Edwards (1970) was used to calculate kinetic parameters-maximum elimination capacity (EC(max)), substrate constant (K(s)), and inhibition constant (K(I)). The model fitted well with the experimental data and the EC(max) was found to be in the range of 10.8-6.1 g/m(3) h. The K(s) values depended upon substrate concentration and ranged from 0.024 to 0.043 g/m(3) indicating the high affinity of diazotrophs for TCE. The K(I) values were low and nearly constant (0.011-0.015 g/m(3)) indicating a moderate substrate inhibition.
研究了填充木屑并接种固氮细菌群落的生物过滤器中 TCE 的生物降解动力学。使用米氏动力学模型表明，该系统存在基质抑制。爱德华兹（1970 年）提出的动力学模型用于计算动力学参数-最大消除容量（EC(max)）、基质常数（K(s)）和抑制常数（K(I)）。该模型很好地拟合了实验数据，发现 EC(max)的范围为 10.8-6.1 g/m(3) h。K(s)值取决于基质浓度，范围为 0.024 至 0.043 g/m(3)，表明固氮菌对 TCE 具有高亲和力。K(I)值较低且几乎恒定（0.011-0.015 g/m(3)），表明存在中等程度的基质抑制。
