Department of Botany, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
Bioresour Technol. 2010 Nov;101(21):8119-26. doi: 10.1016/j.biortech.2010.06.040. Epub 2010 Jul 1.
The biodegradation of TCE was studied in a laboratory scale biofilter packed with wood charcoal and inoculated with mixed culture of methanotrophs isolated from local soil. The removal efficiency was found to be higher than 90% up to an inlet load of 5.1g/m(3)h. The maximum elimination capacity was 6.7g/m(3)h at an inlet loading rate of 11.3g/m(3)h. The reaction constants EC(max,)K(s) and K(i) calculated from the experimental results are also presented. The biodegradation process is found to be inhibited at higher TCE concentration. The carbon dioxide production rate has been found to be a linear function of elimination capacity. The DNA finger printing techniques has indicated the presence of functionally active methanotrophic community including Methylocystis sp. in the biofilter.
研究了在实验室规模的生物过滤器中，用木炭填充并接种了从当地土壤中分离出的甲烷营养菌混合培养物对 TCE 的生物降解。发现去除效率高于 90%，直到入口负荷为 5.1g/m(3)h。在入口负荷率为 11.3g/m(3)h 时，最大消除能力为 6.7g/m(3)h。还介绍了从实验结果计算得出的反应常数 EC(max,)K(s)和 K(i)。发现生物降解过程在较高的 TCE 浓度下受到抑制。二氧化碳的生成速率已被发现是消除能力的线性函数。DNA 指纹图谱技术表明，生物过滤器中存在功能活跃的甲烷营养菌群，包括甲基球菌属。
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