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塞尔维亚伏伊伏丁那省人群线粒体DNA控制区的序列多态性

Sequence polymorphism of the mitochondrial DNA control region in the population of Vojvodina Province, Serbia.

作者信息

Zgonjanin Dragana, Veselinović Igor, Kubat Milovan, Furac Ivana, Antov Mirjana, Loncar Eva, Tasić Milos, Vuković Radenko, Omorjan Radovan

机构信息

Institute of Forensic Medicine, Clinical Center Novi Sad, Hajduk Veljkova 1-7, 21000 Novi Sad, Republic of Serbia.

出版信息

Leg Med (Tokyo). 2010 Mar;12(2):104-7. doi: 10.1016/j.legalmed.2009.10.007. Epub 2009 Dec 4.

DOI:10.1016/j.legalmed.2009.10.007
PMID:19962932
Abstract

In order to generate and establish the database for forensic identification purposes in Vojvodina Province (Serbia), the sequence of the hypervariable regions 1 (HV1) and 2 (HV2) of the mtDNA control region were determined in a population of 104 unrelated individuals from Vojvodina Province, using a fluorescent-based capillary electrophoresis sequencing method. A total of 93 different haplotypes were found, of these 83 mtDNA types were unique, nine haplotypes were shared by two individuals and one haplotype by three individuals. The variation of mtDNA HV1 and HV2 regions was confined to 116 nucleotide positions, of which 72 were observed in the HV1 and 44 in the HV2. A statistical estimate of the results for this population showed the genetic diversity of 0.9977 and the random match probability of 1.18%. Haplogroup H was the most common haplogroup (43.3%). Haplogroups observed at intermediate levels included clusters U (13.5%), T (10.6%), J (8.6%) and W (5.8%).

摘要

为了在塞尔维亚伏伊伏丁那省建立用于法医鉴定目的的数据库,采用基于荧光的毛细管电泳测序方法,对来自伏伊伏丁那省的104名无关个体的线粒体DNA控制区高变区1(HV1)和高变区2(HV2)的序列进行了测定。共发现93种不同的单倍型,其中83种线粒体DNA类型是独特的,9种单倍型由两人共享,1种单倍型由三人共享。线粒体DNA HV1和HV2区域的变异局限于116个核苷酸位置,其中72个在HV1中观察到,44个在HV2中观察到。对该人群结果的统计估计显示,遗传多样性为0.9977,随机匹配概率为1.18%。单倍群H是最常见的单倍群(43.3%)。中等水平观察到的单倍群包括聚类U(13.5%)、T(10.6%)、J(8.6%)和W(5.8%)。

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