Dou Q P, Fridovich-Keil J L, Pardee A B
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA.
Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1157-61. doi: 10.1073/pnas.88.4.1157.
By performing DNase I footprint and band-shift analyses of a 170-base-pair region of the murine thymidine kinase promoter, we identified an inducible DNA binding activity that we named Yi. Yi binding activity was not detected in G0 and G1 extracts, but it was observed as cells crossed the G1/S boundary. Yi proteins bind specifically to a consensus sequence (CCCNCNNNCT) found at three distinct sites in this promoter region. We also observed a murine Sp1 binding activity that was constitutive throughout the cell cycle. We propose that the G1/S-specific Yi binding is important for murine thymidine kinase gene regulation and perhaps also for initiation of DNA synthesis.
通过对小鼠胸苷激酶启动子170个碱基对区域进行DNA酶I足迹和条带迁移分析，我们鉴定出一种可诱导的DNA结合活性，我们将其命名为Yi。在G0期和G1期提取物中未检测到Yi结合活性，但在细胞跨越G1/S边界时可观察到。Yi蛋白特异性结合于该启动子区域三个不同位点发现的共有序列（CCCNCNNNCT）。我们还观察到一种小鼠Sp1结合活性，其在整个细胞周期中都是组成型的。我们提出，G1/S特异性的Yi结合对于小鼠胸苷激酶基因调控很重要，可能对DNA合成的起始也很重要。
