Guhr G, Schmidt H, Weihnacht M
Leibniz Institute for Solid State and Materials Research Dresden, Dresden, Germany.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:729-32. doi: 10.1109/IEMBS.2009.5332908.
In this paper a new tool to assess viscoelastic and dielectric properties of biological samples is presented. Shear horizontal polarized surface acoustic waves (SH-SAW) are used to detect the viscoelastic properties of the extracellular matrix (ECM) expelled by adhering fibroblast cell cultures. Therefore a one-port SAW resonator, with a fundamental mode of 85 MHz was developed. Its electrode structure can be used simultaneously to detect the dielectric behavior of the whole system by impedance spectroscopy (IS) while the frequency ranges from kHz to MHz. The applicability of the combination of both methods appearing as new tool is exemplary shown by cell adhesion experiments performed with L929 and NIH-3T3 fibroblasts.
本文介绍了一种评估生物样品粘弹性和介电特性的新工具。采用水平剪切极化表面声波（SH-SAW）来检测粘附的成纤维细胞培养物排出的细胞外基质（ECM）的粘弹性特性。因此，开发了一种基模为85 MHz的单端口SAW谐振器。其电极结构可同时用于通过阻抗谱（IS）检测整个系统的介电行为，频率范围为kHz至MHz。用L929和NIH-3T3成纤维细胞进行的细胞粘附实验示例性地展示了作为新工具的这两种方法组合的适用性。
