Tandon Nina, Goh Brian, Marsano Anna, Chao Pen-Hsiu Grace, Montouri-Sorrentino Chrystina, Gimble Jeffrey, Vunjak-Novakovic Gordana
Columbia University, Department of Biomedical Engineering, 622 west 168th Street, Vanderbilt Clinic 12-234, New York, NY 10032, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:6517-21. doi: 10.1109/IEMBS.2009.5333142.
In vivo, direct current electric fields are present during embryonic development and wound healing. In vitro, direct current (DC) electric fields induce directional cell migration and elongation. For the first time, we demonstrate that cultured human adipose tissue-derived stem cells (hASCs) respond to the presence of direct-current electric fields. Cells were stimulated for 2-4 hours with DC electric fields of 6 V/cm that were similar to those encountered in vivo post-injury. Upon stimulation, hASCs were observed to elongate and align perpendicularly to the applied electric field, disassemble gap junctions, and upregulate the expression of genes for connexin-43, thrombomodulin, vascular endothelial growth factor, and fibroblast growth factor. In separate related studies, human epicardial fat-derived stem cells (heASCs) were also observed to align and elongate. It is interesting that the morphological and phenotypic characteristics of mesenchymal stem cells derived both from liposuction aspirates and from cardiac fat can be modulated by direct current electric fields. In further studies, we will quantify the effects of the electrical fields in the context of wound healing.
在体内，胚胎发育和伤口愈合过程中存在直流电场。在体外，直流（DC）电场可诱导细胞定向迁移和伸长。我们首次证明，培养的人脂肪组织来源的干细胞（hASC）对直流电场的存在有反应。用6 V/cm的直流电场刺激细胞2 - 4小时，该电场与体内受伤后遇到的电场相似。刺激后，观察到hASC伸长并垂直于施加的电场排列，间隙连接解体，连接蛋白43、血栓调节蛋白、血管内皮生长因子和成纤维细胞生长因子的基因表达上调。在单独的相关研究中，也观察到人心外膜脂肪来源的干细胞（heASC）排列并伸长。有趣的是，抽脂吸出物和心脏脂肪来源的间充质干细胞的形态和表型特征都可被直流电场调节。在进一步的研究中，我们将在伤口愈合的背景下量化电场的作用。
