Mitrea Bogdan G, Wellner Marcel, Pertsov Arkady M
State University of New York, Upstate Medical University, Syracuse, NY 13210, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:4194-7. doi: 10.1109/IEMBS.2009.5334048.
Intramyocardial reentry is implicated as a primary cause of the most deadly cardiac arrhythmias known as polymorphic ventricular tachycardia and ventricular fibrillation. However, the mechanisms involved in the triggering of such reentry and controlling its subsequent dynamics remain poorly understood. One of the major obstacles has been a lack of adequate tools that would enable 3D imaging of electrical excitation and reentry inside thick ventricular wall. Here, we present a new experimental technique, termed alternating transillumination (AT), aimed at filling this gap. The AT technique utilizes a recently synthesized near-infrared fluorescent voltage-sensitive dye, DI-4-ANBDQBS. We apply AT to study the dynamics of reentry during shock-induced polymorphic ventricular tachycardia in pig myocardium.
心肌内折返被认为是最致命的心律失常——多形性室性心动过速和心室颤动的主要原因。然而，引发这种折返并控制其后续动态的机制仍知之甚少。主要障碍之一是缺乏足够的工具来对厚心室壁内的电兴奋和折返进行三维成像。在此，我们提出一种新的实验技术，称为交替透照（AT），旨在填补这一空白。AT技术利用了最近合成的近红外荧光电压敏感染料DI-4-ANBDQBS。我们应用AT来研究猪心肌中电击诱发的多形性室性心动过速期间折返的动态变化。
