Department of Biochemistry and Biophysics, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California, USA.
Nat Methods. 2010 Jan;7(1):50-2. doi: 10.1038/nmeth.1406. Epub 2009 Dec 6.
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.
我们描述了一种在小鼠胚胎干细胞（ESCs）中高效、精确靶向修饰基因陷阱基因座的方法。通过 Floxin 方法，利用 Cre 重组酶和穿梭载体 pFloxin，可使基因陷阱突变逆转并插入新的 DNA 序列。Floxin 技术适用于现有的 24,149 个兼容基因陷阱细胞系，这应该能够实现小鼠 ESCs 中许多基因的高通量修饰。
