Lima C C, Pereira A P C, Silva J R F, Oliveira L S, Resck M C C, Grechi C O, Bernardes M T C P, Olímpio F M P, Santos A M M, Incerpi E K, Garcia J A D
Universidade José do Rosário Vellano, Alfenas, MG, Brazil.
Braz J Biol. 2009 Nov;69(4):1195-201. doi: 10.1590/s1519-69842009000500026.
Healing is a complex process that involves cellular and biochemical events. Several medicines have been used in order to shorten healing time and avoid aesthetic damage.
to verify the topical effect of ascorbic acid for the healing of rats' skin wounds through the number of macrophages, new vessels and fibroblast verifications in the experimental period; and analyse the thickness and the collagen fibre organization in the injured tissue.
Male Rattus norvegicus weighing 270 +/- 30 g were used. After thionembutal anesthesia, 15 mm transversal incisions were made in the animals' cervical backs. They were divided into two groups: Control Group (CG, n = 12) - skin wound cleaned with water and soap daily; Treated Group (TG, n = 12) - skin wound cleaned daily and treated with ascorbic acid cream (10%). Samples of skin were collected on the 3rd, 7th and 14th days. The sections were stained with hematoxylin-eosin and picrosirius red for morphologic analysis. The images were obtained and analysed by a Digital Analyser System.
The ascorbic acid acted on every stage of the healing process. It reduced the number of macrophages, increased the proliferation of fibroblasts and new vessels, and stimulated the synthesis of thicker and more organized collagen fibres in the wounds when compared to CG.
Ascorbic acid was shown to have anti-inflammatory and healing effects, guaranteeing a suiTable environment and conditions for faster skin repair.
愈合是一个涉及细胞和生化事件的复杂过程。为了缩短愈合时间并避免美学损伤，人们使用了多种药物。
通过在实验期间对巨噬细胞、新血管和成纤维细胞数量的验证，来验证抗坏血酸对大鼠皮肤伤口愈合的局部作用；并分析损伤组织中的厚度和胶原纤维组织。
使用体重为270±30克的雄性褐家鼠。经硫喷妥钠麻醉后，在动物的颈背部做15毫米的横向切口。将它们分为两组：对照组（CG，n = 12）——每天用水和肥皂清洁皮肤伤口；治疗组（TG，n = 12）——每天清洁皮肤伤口并用抗坏血酸乳膏（10%）治疗。在第3天、第7天和第14天采集皮肤样本。切片用苏木精-伊红和苦味酸天狼星红染色进行形态学分析。通过数字分析仪系统获取并分析图像。
抗坏血酸作用于愈合过程的每个阶段。与对照组相比，它减少了巨噬细胞数量，增加了成纤维细胞和新血管的增殖，并刺激伤口中更厚且更有组织的胶原纤维的合成。
抗坏血酸具有抗炎和愈合作用，为更快的皮肤修复保证了合适的环境和条件。
