Colombo Maria Laura, Bugatti Carlo, Davanzo Franca, Persico Andrea, Ballabio Cinzia, Restani Patrizia
Department of Drug & Science Technology, University of Torino, Torino, Italy.
Nat Prod Commun. 2009 Nov;4(11):1551-2.
A sensitive and specific method for aconitine extraction from biological samples was developed. Aconitine, the main toxic alkaloid from plants belonging to Aconitum species (family Ranunculaceae), was determined in plant material by an external standard method, and by a standard addition calibration method in biological fluids. Described here is one fatal case and five intoxications of accidental aconitine poisoning following the ingestion of aconite mistaken for an edible grass, Aruncus dioicus (Walt.) Fernald, "mountain asparagus", and Cicerbita alpina (L.) Wallroth. The aconitine content in urine was in the range 2.94 microg/mL (dead patient)-0.20 microg/mL (surviving patients), which was almost two to four times higher than that in plasma.
开发了一种从生物样品中提取乌头碱的灵敏且特异的方法。乌头碱是毛茛科乌头属植物中的主要有毒生物碱，通过外标法在植物材料中测定，在生物体液中则采用标准加入校准法测定。本文描述了1例致命病例以及5例因误食误认为是可食用草类的乌头、假升麻（“山芦笋”）和高山苦苣菜而意外发生乌头碱中毒的案例。尿液中的乌头碱含量范围为2.94微克/毫升（死亡患者）至0.20微克/毫升（存活患者），几乎比血浆中的含量高出两到四倍。
