Martin-Alguacil N, Babich H, Rosenberg D W, Borenfreund E
Laboratory Animal Research Center, Rockefeller University, New York, New York 10021.
Arch Environ Contam Toxicol. 1991 Jan;20(1):113-7. doi: 10.1007/BF01065336.
Established cell lines from brown bullhead catfish (BB) and rainbow trout (RTG-2) and primary cultures of cells derived from gill, fin, and gonad tissues from brown bullhead catfish were evaluated for use as bioindicators in the neutral red cytotoxicity assay. The BB and RTG-2 cells were compared after a 1 day exposure to chlorinated pesticides and after a 6-day exposure to various polycyclic aromatic hydrocarbons. The BB cells were more sensitive to both classes of chemicals. The sequence of toxicity for the BB cells was 4,4'-DDD. 4,4'-DDT greater than aldrin, 4,4'-DDE and 7,12-dimethylbenz(a)anthracene (DMBA) greater than 3-hydroxybenzo(a)pyrene (3-OH-B(a)P) greater than benzo(a)pyrene (B(a)P). For the RTG-2 cells, the sequence was aldrin greater than 4,4'-DDD greater than 4,4'-DDT greater than 4,4'-DDE and 3-OH-B(a)P greater than DMBA greater than B(a)P. The BB cells were also sensitive to benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)anthracene, and trans-7,8-dihydro-7,8-dihydroxybenzo(a)pyrene. The responses of BB and RTG-2 cells were compared with those of primary cultures after a 1 day exposure to B(a)P. After 1 day of exposure, the RTG-2 cells and primary cultures were more sensitive than the BB cells to B(a)P. Apparently, after 1 day of incubation the RTG-2 and primary cells metabolized greater amounts of B(a)P to cytotoxic metabolites, than did the BB cells. However, by 6 days of incubation, the BB cells were more sensitive to B(a)P than were the RTG-2 cells. A 6-day exposure to B(a)P was not performed with the primary cell cultures.
对来自褐首鲶（BB）和虹鳟（RTG - 2）的已建立细胞系，以及源自褐首鲶鳃、鳍和性腺组织的原代细胞培养物进行了评估，以确定它们在中性红细胞毒性试验中作为生物指示剂的用途。在暴露于氯化农药1天以及暴露于各种多环芳烃6天后，对BB和RTG - 2细胞进行了比较。BB细胞对这两类化学物质更敏感。BB细胞的毒性顺序为4,4'-滴滴滴＞4,4'-滴滴涕＞艾氏剂、4,4'-滴滴伊和7,12 - 二甲基苯并(a)蒽（DMBA）＞3 - 羟基苯并(a)芘（3 - OH - B(a)P）＞苯并(a)芘（B(a)P）。对于RTG - 2细胞，顺序为艾氏剂＞4,4'-滴滴滴＞4,4'-滴滴涕＞4,4'-滴滴伊和3 - OH - B(a)P＞DMBA＞B(a)P。BB细胞对苯并(b)荧蒽、苯并(k)荧蒽、苯并(a)蒽和反式 - 7,8 - 二氢 - 7,8 - 二羟基苯并(a)芘也敏感。在暴露于B(a)P 1天后，将BB和RTG - 2细胞的反应与原代培养物的反应进行了比较。暴露1天后，RTG - 2细胞和原代培养物对B(a)P比BB细胞更敏感。显然，孵育1天后，RTG - 2细胞和原代细胞比BB细胞将更多量的B(a)P代谢为细胞毒性代谢物。然而，到孵育6天时，BB细胞对B(a)P比RTG - 2细胞更敏感。原代细胞培养物未进行6天的B(a)P暴露。
