Laboratory of Neuroscience, Faculty of Psychology, University of Oviedo, Plaza Feijoo, s/n E-33003, Oviedo, Spain.
Neurobiol Learn Mem. 2010 Mar;93(3):362-71. doi: 10.1016/j.nlm.2009.12.002. Epub 2009 Dec 5.
The progression of brain circuits involved in spatial learning tasks is still a matter of debate. In addition, the participation of individual regions at different stages of spatial learning remains a controversial issue. In order to address these questions, we used quantitative cytochrome oxidase histochemistry as a metabolic brain mapping method applied to rats (Rattus norvegicus) trained in a water maze for 1, 3 or 5 days of training. Sustained changes throughout training were found in the lateral septal nucleus and anteroventral thalamic nucleus. As compared to naïve or habituation groups, rats with 1 day of training in the spatial learning task showed involvement of the lateral mammillary nucleus, basolateral amygdala and anterodorsal thalamic nucleus. By 5 days of training, there were mean changes in the hippocampal CA3 field and the prefrontal cortex. The regions involved and their pattern of network interactions changed progressively over days of training. At 1-day there was an open serial network of pairwise correlations. At 3-days there was a more closed reciprocal network of intercorrelations. At 5-days there were three separate parallel networks. In addition, brain-behavior correlations showed that CA1 and CA3 hippocampal fields together with the parietal cortex are related to the mastery of the spatial learning task. The present study extends previous findings on the progressive contribution of neural networks to spatial learning.
参与空间学习任务的脑回路的进展仍然存在争议。此外，个体区域在空间学习的不同阶段的参与仍然是一个有争议的问题。为了解决这些问题，我们使用定量细胞色素氧化酶组织化学作为代谢脑图谱方法，应用于在水迷宫中接受 1、3 或 5 天训练的大鼠（Rattus norvegicus）。在整个训练过程中，发现外侧隔核和前腹侧丘脑核发生了持续变化。与未经训练或习惯化的对照组相比，在空间学习任务中接受 1 天训练的大鼠显示出外侧乳突核、基底外侧杏仁核和前背侧丘脑核的参与。经过 5 天的训练，海马 CA3 区和前额叶皮质发生了平均变化。参与的区域及其网络相互作用的模式随着训练天数的增加而逐渐变化。在 1 天，存在开放的串行网络对相关。在 3 天，存在更封闭的相互相关网络。在 5 天，存在三个独立的平行网络。此外，脑行为相关性表明，海马 CA1 和 CA3 区与顶叶皮层共同与空间学习任务的掌握有关。本研究扩展了关于神经网络对空间学习的渐进贡献的先前发现。
