RELT 诱导 HEK 293 上皮细胞死亡。
RELT induces cellular death in HEK 293 epithelial cells.
机构信息
University of Colorado Denver School of Dental Medicine, Department of Craniofacial Biology, 12801 East 17th Avenue, Aurora, CO 80045, USA.
出版信息
Cell Immunol. 2010;261(1):1-8. doi: 10.1016/j.cellimm.2009.10.013. Epub 2009 Nov 4.
Abstract
RELT is a recently identified Tumor Necrosis Factor Receptor that possess two homologues in humans named RELL1 and RELL2. We investigated whether RELT and its homologues could induce cellular death when transiently transfected into HEK 293 epithelial cells. Transfection of RELT family members into HEK 293 epithelial cells induced cell death characterized by rounding and lifting of cells accompanied by DNA fragmentation, characteristics that are consistent with the activation of an apoptotic pathway. Overexpression of RELT in COS-7 cells resulted in cell rounding and lifting without DNA fragmentation, suggesting that the effects of RELT signaling may vary among different cell types. In summary, we report that overexpression of RELT or its homologues RELL1 and RELL2 in HEK 293 epithelial cells results in cell death with morphological characteristics consistent with the activation of an apoptotic pathway.
摘要
RELT 是一种新鉴定的肿瘤坏死因子受体，在人类中拥有两个同源物，分别命名为 RELL1 和 RELL2。我们研究了 RELT 及其同源物在瞬时转染 HEK 293 上皮细胞时是否会诱导细胞死亡。RELT 家族成员转染 HEK 293 上皮细胞会诱导细胞死亡，其特征是细胞变圆和提起，同时伴随着 DNA 片段化，这些特征与凋亡途径的激活一致。在 COS-7 细胞中过表达 RELT 会导致细胞变圆和提起，但不会导致 DNA 片段化，这表明 RELT 信号的作用可能在不同的细胞类型中有所不同。总之，我们报告称，在 HEK 293 上皮细胞中过表达 RELT 或其同源物 RELL1 和 RELL2 会导致细胞死亡，其形态学特征与凋亡途径的激活一致。
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Biochem Biophys Res Commun. 2006 Apr 28;343(1):125-34. doi: 10.1016/j.bbrc.2006.02.125. Epub 2006 Mar 2.
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