Aubut Virginie, Pommel Ludovic, Verhille Bernard, Orsière Thierry, Garcia Serge, About Imad, Camps Jean
Laboratoire Interface Matrice Extracellulaire Biomatériaux, Faculté d'Odontologie, Marseille, France.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Feb;109(2):e120-5. doi: 10.1016/j.tripleo.2009.09.022. Epub 2009 Dec 6.
The objective of this study was to evaluate the influence of neutralizing a 2.5% NaOCl solution on its cytotoxicity, genotoxicity, and tissue-dissolving potential.
The cytotoxicity and the genotoxicity of Dakin, a 2.5% NaOCl solution, and a neutralized 2.5% NaOCl solution were assessed according to ISO 10993 standards. The weight of palatal mucosa samples placed in neutralized 2.5% NaOCl, 2.5% NaOCl was recorded over time as well as the pH of the solutions.
The neutralized 2.5% NaOCl solution was 10-fold more cytotoxic than the 2.5% NaOCl solution. None of the solutions was genotoxic. The 2.5% NaOCl solution had a better tissue-dissolving capacity than the neutralized 2.5% NaOCl solution. The pH of the 2.5% NaOCl solution and neutralized 2.5% NaOCl solution decreased from 12 to 9 and from 7.5 to 5.6, respectively.
Neutralizing a 2.5% NaOCl solution increased its cytotoxicity, did not induce any genotoxic effect, and reduced its tissue-dissolving ability.
本研究的目的是评估中和2.5%次氯酸钠溶液对其细胞毒性、遗传毒性和组织溶解能力的影响。
根据ISO 10993标准评估达金溶液、2.5%次氯酸钠溶液和中和后的2.5%次氯酸钠溶液的细胞毒性和遗传毒性。记录放置在中和后的2.5%次氯酸钠溶液、2.5%次氯酸钠溶液中的腭黏膜样本随时间的重量以及溶液的pH值。
中和后的2.5%次氯酸钠溶液的细胞毒性比2.5%次氯酸钠溶液高10倍。所有溶液均无遗传毒性。2.5%次氯酸钠溶液比中和后的2.5%次氯酸钠溶液具有更好的组织溶解能力。2.5%次氯酸钠溶液和中和后的2.5%次氯酸钠溶液的pH值分别从12降至9和从7.5降至5.6。
中和2.5%次氯酸钠溶液会增加其细胞毒性，不会诱导任何遗传毒性作用，并降低其组织溶解能力。
