Leonard B A, Lee P K, Jenkins M K, Schlievert P M
Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455.
Infect Immun. 1991 Mar;59(3):1210-4. doi: 10.1128/iai.59.3.1210-1214.1991.
Streptococcal pyrogenic exotoxins (SPEs) A, B, and C, like other members of the pyrogenic toxin family, are able to cause toxic shock-like syndromes. One of the major properties of these toxins is the ability to induce T-cell proliferation. Characterization of T cell mitogenicity associated with SPEs A, B, and C was undertaken. SPEs A, B, and C were mitogenic for C57BL10/SnJ and BALB/cWAT T cells, with activities differing in intensity depending on the mouse strain and toxin employed. SPE-induced, T-cell-proliferative activity was dependent on class II major histocompatibility complex molecules expressed on antigen-presenting cells. The abilities of SPEs A, B, and C to preferentially stimulate murine cells with certain T-cell receptor V beta s were investigated by fluorescence-activated cell sorter analysis. SPE A preferentially activated T cells expressing V beta 8 but not V beta 3, 6, or 11, while SPEs B and C preferentially stimulated T cells which did not express any of the tested V beta s.
链球菌致热外毒素（SPEs）A、B和C与致热毒素家族的其他成员一样，能够引起中毒性休克样综合征。这些毒素的主要特性之一是诱导T细胞增殖的能力。我们对与SPEs A、B和C相关的T细胞促有丝分裂活性进行了表征。SPEs A、B和C对C57BL10/SnJ和BALB/cWAT T细胞具有促有丝分裂作用，其活性强度因小鼠品系和所用毒素而异。SPE诱导的T细胞增殖活性依赖于抗原呈递细胞上表达的II类主要组织相容性复合体分子。通过荧光激活细胞分选分析研究了SPEs A、B和C优先刺激具有某些T细胞受体Vβs的小鼠细胞的能力。SPE A优先激活表达Vβ8的T细胞，而不激活Vβ3、6或11，而SPEs B和C优先刺激不表达任何测试Vβs的T细胞。
