Thirkell D, Myles A D, Russell W C
Department of Biochemistry and Microbiology, University of St. Andrews, Fife Scotland.
Infect Immun. 1991 Mar;59(3):781-4. doi: 10.1128/iai.59.3.781-784.1991.
After incubation of Ureaplasma urealyticum serotype 8 in the presence of 3H-labeled palmitic acid, about 25 acylated proteins were detected by electrophoresis and fluorography. Of these, at least six were shown to be antigenic by immunoprecipitation of solubilized palmitate-labeled cells with a homologous polyclonal serum. These six included the serotype 8-specific surface-expressed 96-kDa antigen. After phase partition of palmitate-labeled cells with Triton X-114, all but six acylated proteins partitioned entirely into the detergent phase. The others, including the 96-kDa antigen, partitioned preferentially into the detergent phase and were apparently amphipathic. These results are consistent with the acylated proteins being mainly membrane associated.
将解脲脲原体8型在3H标记的棕榈酸存在下培养后，通过电泳和荧光自显影检测到约25种酰化蛋白。其中，用同源多克隆血清对溶解的棕榈酸酯标记细胞进行免疫沉淀，结果显示至少六种具有抗原性。这六种蛋白包括8型特异性表面表达的96 kDa抗原。用Triton X-114对棕榈酸酯标记细胞进行相分离后，除六种酰化蛋白外，其余全部进入去污剂相。其他蛋白，包括96 kDa抗原，优先进入去污剂相，显然具有两亲性。这些结果与酰化蛋白主要与膜相关一致。
