Cogen B, Eckhart W
J Virol. 1977 Nov;24(2):701-3. doi: 10.1128/JVI.24.2.701-703.1977.
We measured the accumulation of virus-specific early RNA in BALB/3T3 cells infected by the ts3 mutant of polyoma virus by annealing cytoplasmic RNA from infected cells to purified, radiolabeled, "early" strand of polyoma DNA. Cells infected by the ts3 mutant fail to accumulate virus-specific early RNA at the nonpermissive temperature.
我们通过将感染细胞的细胞质RNA与纯化的、放射性标记的多瘤病毒“早期”DNA链退火,来测量被多瘤病毒ts3突变体感染的BALB/3T3细胞中病毒特异性早期RNA的积累情况。在非允许温度下,被ts3突变体感染的细胞无法积累病毒特异性早期RNA。