多瘤病毒DNA中基因功能的定位

Feunteun J, Sompayrac L, Fluck M, Benjamin T

Proc Natl Acad Sci U S A. 1976 Nov;73(11):4169-73. doi: 10.1073/pnas.73.11.4169.

Polyoma virus mutants of four functionally distinct groups have been mapped by the marker rescue technique using restriction enzyme fragments of wild-type viral DNA. Nontransforming host-range mutants map in the proximal part of the early region of the viral genome. The same DNA fragment that restores a normal host range also restores normal transforming ability to these mutants. ts-25D, a temperature-sensitive (ts)-a class mutant, maps in the distal part of the early region. ts-3 and ts-1260 map in the proximal and distal parts of the late region, respectively.

利用野生型病毒DNA的限制性酶切片段，通过标记拯救技术对四个功能不同组的多瘤病毒突变体进行了定位。非转化宿主范围突变体定位于病毒基因组早期区域的近端部分。恢复正常宿主范围的同一DNA片段也恢复了这些突变体的正常转化能力。ts-25D是一种温度敏感（ts）-a类突变体，定位于早期区域的远端部分。ts-3和ts-1260分别定位于晚期区域的近端和远端部分。