Uedelhoven W M, Rützel A, Meese C O, Weber P C
Institut für die Prophylaxe und Epidemilogie der Kreislauferkrankungen b.d. Universität München, F.R.G.
Biochim Biophys Acta. 1991 Jan 28;1081(2):197-201. doi: 10.1016/0005-2760(91)90026-e.
Chronic smoking is a major risk factor of atherosclerosis and coronary heart disease. The measurement of three major thromboxane A2 metabolites, 11-dehydrothromboxane B2, 2,3-dinorthromboxane B2 and thromboxane B2, in the urines of 13 apparently healthy smokers (average 39 years, range 27-56 years) showed significantly elevated excretion rates for all thromboxane A2 metabolites as compared to 10 apparently healthy age-matched non-smokers (average 37 years, range 26-56 years). Importantly, characteristic alterations in the thromboxane A2 metabolite pattern were found in the urines of smokers. The contribution of 2,3-dinorthromboxane B2 to total measured excretion of thromboxane A2 metabolites was 59.2% in smokers (404.0 +/- 53.0 pg/mg creatinine) versus 19.4% in non-smokers (85.2 +/- 8.3 pg/mg creatinine), that of 11-dehydrothromboxane B2 35.7% in smokers (673.2 +/- 88.9 pg/mg creatinine) as compared to 75.5% in non-smokers (332.6 +/- 30.9 pg/mg creatinine). The contribution of thromboxane B2 (57.5 +/- 7.7 pg/mg creatinine in smokers versus 21.9 +/- 1.5 pg/mg creatinine in non-smokers) was similar at 5.1%. The excretion of cotinine, the major urinary metabolite of nicotine that correlates well with the reported daily cigarette consumption (r = 0.97, P less than 0.0001), showed a good correlation to thromboxane A2 metabolite excretion (2,3-dinorthromboxane B2: r = 0.92, P less than 0.0001; 11-dehydrothromboxane B2; r = 0.87, P less than 0.0001).
长期吸烟是动脉粥样硬化和冠心病的主要危险因素。对13名表面健康的吸烟者（平均39岁，年龄范围27 - 56岁）尿液中三种主要血栓素A2代谢物，即11 - 脱氢血栓素B2、2,3 - 二去甲血栓素B2和血栓素B2的测量显示，与10名年龄匹配的表面健康的非吸烟者（平均37岁，年龄范围26 - 56岁）相比，所有血栓素A2代谢物的排泄率均显著升高。重要的是，在吸烟者尿液中发现了血栓素A2代谢物模式的特征性改变。吸烟者尿液中2,3 - 二去甲血栓素B2占血栓素A2代谢物总排泄量的比例为59.2%（404.0±53.0 pg/mg肌酐），而非吸烟者为19.4%（85.2±8.3 pg/mg肌酐）；吸烟者尿液中11 - 脱氢血栓素B2占35.7%（673.2±88.9 pg/mg肌酐），非吸烟者为75.5%（332.6±30.9 pg/mg肌酐）。血栓素B2的比例（吸烟者为57.5±7.7 pg/mg肌酐，非吸烟者为21.9±1.5 pg/mg肌酐）相似，为5.1%。可替宁是尼古丁的主要尿液代谢物，其排泄与报告的每日吸烟量密切相关（r = 0.97，P < 0.0001），与血栓素A2代谢物排泄也显示出良好的相关性（2,3 - 二去甲血栓素B2：r = 0.92，P < 0.0001；11 - 脱氢血栓素B2：r = 0.87，P < 0.0001）。
