Cusick E L, Ewen S W, Krukowski Z H, Matheson N A
Department of Surgery, University of Aberdeen, UK.
Br J Surg. 1991 Jan;78(1):94-6. doi: 10.1002/bjs.1800780129.
The potential value of DNA aneuploidy, in distinguishing benign from malignant follicular thyroid neoplasms, was studied. The nuclear DNA content of 65 follicular thyroid neoplasms (52 adenomas and 13 carcinomas) was determined by flow cytometric analysis of paraffin embedded material; in 58 cases preparations were technically satisfactory. In 22 follicular neoplasms DNA analysis was also performed on fresh material obtained by fine needle aspiration of surgical specimens. Cell cycle analysis was performed on both fresh and fixed specimens. An aneuploid DNA profile was found on analysis of fixed tissue in eight of 45 (18 per cent) follicular adenomas and four of 13 (31 per cent) follicular carcinomas. DNA aneuploidy was also found in six of the 22 (27 per cent) fresh preparations from follicular adenomas. The frequency of DNA aneuploidy in apparently benign and malignant follicular neoplasms was similar. Follicular thyroid neoplasia are best regarded as a single entity with a low incidence of local and distant spread. All follicular neoplasia are therefore best excised.
研究了DNA非整倍体在鉴别甲状腺滤泡性肿瘤良恶性方面的潜在价值。通过对石蜡包埋材料进行流式细胞术分析，测定了65例甲状腺滤泡性肿瘤（52例腺瘤和13例癌）的核DNA含量；58例标本的技术处理结果令人满意。对22例滤泡性肿瘤，还对手术标本细针穿刺获取的新鲜材料进行了DNA分析。对新鲜标本和固定标本均进行了细胞周期分析。在45例滤泡性腺瘤中的8例（18%）和13例滤泡性癌中的4例（31%）的固定组织分析中发现了非整倍体DNA图谱。在22例滤泡性腺瘤新鲜标本中的6例（27%）也发现了DNA非整倍体。明显良性和恶性的滤泡性肿瘤中DNA非整倍体的频率相似。甲状腺滤泡性肿瘤最好被视为一个单一实体，局部和远处转移发生率低。因此，所有甲状腺滤泡性肿瘤最好都予以切除。
