Haruna Y, Hayashi N, Katayama K, Yuki N, Kasahara A, Sasaki Y, Fusamoto H, Kamada T
First Department of Medicine, Osaka University Medical School, Japan.
Hepatology. 1991 Mar;13(3):417-21.
The X protein can act on the enhancer of hepatitis B virus in an in vitro system and elevate the transcriptional level of hepatitis B virus. However, because no relationship had been reported between X protein expression and hepatitis B virus replication in patients with chronic hepatitis B, we focused on its expression in the liver in comparison with markers of hepatitis B virus replication. Liver biopsy samples and sera from 59 carriers with HBsAg were examined immunohistochemically for X protein using rabbit IgG against recombinant X protein. There was a significant difference in the serum hepatitis B virus DNA level between X protein-positive and -negative patients (p less than 0.001). Serum pre-S1 and pre-S2 antigens were also measured quantitatively by enzyme immunoassay using monoclonal antibodies specific against each antigen. The titers of pre-S1 antigen in patients positive for X protein were significantly higher (p less than 0.001) than those of the X protein-negative patients (3.02 +/- 0.99 vs. 2.00 +/- 0.59, respectively). Similarly, the titers of pre-S2 antigen were 2.98 +/- 0.91 vs. 1.94 +/- 0.54, respectively (p less than 0.001). The rate of positivity of the X protein was higher (38 of 49; 77.6%) in the replicative group (serum HBeAg, serum hepatitis B virus DNA or HBcAg in liver positive) compared with that observed in the nonreplicative group (3 of 10; 30%--serum HBeAg, serum hepatitis B virus DNA and HBcAg in liver negative) (p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
X蛋白可在体外系统中作用于乙肝病毒增强子，提高乙肝病毒转录水平。然而，由于慢性乙肝患者中X蛋白表达与乙肝病毒复制之间的关系此前未见报道，我们将其在肝脏中的表达与乙肝病毒复制标志物进行了比较。采用抗重组X蛋白的兔IgG，对59例HBsAg携带者的肝活检样本和血清进行X蛋白免疫组化检测。X蛋白阳性和阴性患者的血清乙肝病毒DNA水平存在显著差异（p<0.001）。还采用针对各抗原的单克隆抗体，通过酶免疫测定法定量检测血清前S1和前S2抗原。X蛋白阳性患者的前S1抗原滴度显著高于X蛋白阴性患者（分别为3.02±0.99和2.00±0.59，p<0.001）。同样，前S2抗原滴度分别为2.98±0.91和1.94±0.54（p<0.001）。与非复制组（10例中有3例，30%，血清HBeAg、血清乙肝病毒DNA和肝脏HBcAg均为阴性）相比，复制组（血清HBeAg、血清乙肝病毒DNA或肝脏HBcAg阳性）中X蛋白阳性率更高（49例中有38例，77.6%）（p<0.01）。（摘要截断于250字）
