Salhanick A I, Schwartz S I, Amatruda J M
Department of Medicine, University of Rochester School of Medicine and Dentistry, NY 14642.
Metabolism. 1991 Mar;40(3):275-9. doi: 10.1016/0026-0495(91)90109-a.
The effect of insulin on apolipoprotein (apo) B secretion was investigated in human hepatocytes. Freshly isolated hepatocytes, prepared by collagenase dispersion of liver specimens, were incubated in serum-free media in the absence and presence of 100 nmol/L insulin for 2 hours. The media was then assayed for apo B content by radioimmunoassay. In hepatocytes incubated without insulin, the secretion of apo B (relative to human low-density lipoprotein [LDL]) was 125 +/- 37 ng/10(6) cells/2 hours. In the presence of insulin, apo B secretion was reduced to 83 +/- 29 ng/10(6) cells/2 hours (34% inhibition, P less than .05). These results using human hepatocytes are consistent with previous data from our laboratory describing insulin-dependent inhibition of apo B secretion in primary cultures of rat hepatocytes and studies by others employing the human-derived hepatoma cell line, Hep G2. We conclude that human hepatic apo B secretion is under insulin control. The role of more chronic insulin exposure requires further investigation.
在人肝细胞中研究了胰岛素对载脂蛋白（apo）B分泌的影响。通过用胶原酶分散肝脏标本制备的新鲜分离的肝细胞，在无血清培养基中分别于不存在和存在100 nmol/L胰岛素的情况下孵育2小时。然后通过放射免疫测定法检测培养基中的apo B含量。在无胰岛素孵育的肝细胞中，apo B（相对于人低密度脂蛋白[LDL]）的分泌为125±37 ng/10⁶细胞/2小时。在有胰岛素存在的情况下，apo B分泌降至83±29 ng/10⁶细胞/2小时（抑制34%，P<0.05）。这些用人肝细胞得到的结果与我们实验室先前描述胰岛素依赖性抑制大鼠肝细胞原代培养物中apo B分泌的数据以及其他人用人源肝癌细胞系Hep G2进行的研究结果一致。我们得出结论，人肝脏apo B分泌受胰岛素控制。更长期胰岛素暴露的作用需要进一步研究。
