Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea.
Environ Sci Technol. 2009 Dec 15;43(24):9335-40. doi: 10.1021/es902407g.
Single-stranded DNA aptamers were generated from a random library to remove arsenic from Vietnamese groundwater. On the basis of significant arsenic contamination levels, three areas in Ha Nam province (Vinh Tru, Bo De, and Hoa Hau) and five areas near the Mekong River Delta (MR1-5) were selected as study areas. The aptamers were in vitro selected using an arsenic aptamer affinity column created by immobilizing arsenic on Affi-gel 10 resin. Quantitative analyses of the aptamer candidates Ars-1 to Ars-8 by surface plasmon resonance (SPR) revealed the Ars-3 aptamer to have the highest affinity to arsenate [(As(V)] and arsenite [As(III)] with a dissociation constant (K(d)) of 4.95 +/- 0.31 and 7.05 +/- 0.91 nM, respectively. The specific affinity interactions of the Ars-3 aptamer to arsenic were verified against other heavy metals. After obtaining successful removal results with a laboratory-prepared aqueous arsenic solution, Ars-3 was applied for removal of any arsenic present in the groundwater samples collected from the studied areas in Vietnam. Field results were also successful: various arsenic concentrations ranging from 28.1 to 739.2 microg/L were completely removed after 5 min of incubation with the arsenic-binding aptamer Ars-3.
从一个随机文库中生成了单链 DNA 适体，以从越南地下水中去除砷。鉴于砷的严重污染水平，选择了海阳省的三个地区（Vinh Tru、Bo De 和 Hoa Hau）和湄公河三角洲附近的五个地区（MR1-5）作为研究区域。通过将砷固定在 Affi-gel 10 树脂上，创建了砷适体亲和柱，在体外对适体进行了选择。表面等离子体共振（SPR）对适体候选物 Ars-1 至 Ars-8 的定量分析表明，Ars-3 适体对砷酸盐[(As(V)]和亚砷酸盐[As(III)]具有最高的亲和力，解离常数（K(d)）分别为 4.95 +/- 0.31 和 7.05 +/- 0.91 nM。Ars-3 适体对砷的特异性亲和相互作用得到了对其他重金属的验证。在用实验室制备的含砷水溶液获得成功去除结果后，将 Ars-3 应用于去除从越南研究地区采集的地下水样品中存在的任何砷。野外结果也取得了成功：用砷结合适体 Ars-3 孵育 5 分钟后，各种浓度为 28.1 至 739.2 µg/L 的砷完全被去除。
