Wanchu Ajay, Rana S V, Pallikkuth Suresh, Sachdeva Ravinder Kaur
Department of Internal Medicine and Gastroenterology, PGIMER, Chandigarh, India.
AIDS Res Hum Retroviruses. 2009 Dec;25(12):1307-11. doi: 10.1089/aid.2009.0062.
HIV infection increases the oxidative stress process, and antiretroviral combination therapy increases protein oxidation and preexistent oxidative stress. The latter induces production of reactive oxygen species. Lipid peroxidation (LPO) is a means of determining oxidative stress. There is also a deficiency of glutathione in HIV infection. Persistent oxidative load leads to an accelerated rate of consumption of glutathione (GSH). This study measured LPO and GSH levels in plasma of HIV-infected individuals with or without therapy and compared these with healthy controls. One hundred HIV-infected individuals and 30 healthy controls were included in the study. LPO and GSH levels were measured in plasma according to previously described methods. The mean level of LPO in HIV-infected individuals was 0.7 +/- 0.1 micromol/ml (range, 0.5-0.9 micromol/ml), whereas the mean LPO level in controls was 0.3 +/- 0.1 micromol/ml (range, 0.2-0.4 micromol/ml). The mean LPO levels were significantly higher in HIV-infected individuals as compared to healthy controls (p value <0.0001). The mean GSH level in HIV-infected individuals was 0.06 +/- 0.01 micromol/ml (range, 0.03-0.08). The mean GSH level in healthy controls was 0.09 +/- 0.01 micromol/ml (range, 0.05-0.1). The mean glutathione level in HIV-infected individuals was significantly lower in compared to healthy controls (p value < 0.0001). There was a significant positive correlation between absolute CD4 cells and GSH levels (rho = 0.182, p = 0.045). There is increased oxidative stress in HIV-infected patients. Whether supplementation with antioxidants will reduce this oxidative stress is still unknown.
HIV感染会加剧氧化应激过程，而抗逆转录病毒联合疗法会增加蛋白质氧化及先前已存在的氧化应激。后者会诱导活性氧的产生。脂质过氧化（LPO）是测定氧化应激的一种方法。HIV感染还存在谷胱甘肽缺乏的情况。持续的氧化负荷会导致谷胱甘肽（GSH）的消耗速率加快。本研究测定了接受或未接受治疗的HIV感染者血浆中的LPO和GSH水平，并将其与健康对照者进行比较。该研究纳入了100名HIV感染者和30名健康对照者。根据先前描述的方法测定血浆中的LPO和GSH水平。HIV感染者的LPO平均水平为0.7±0.1微摩尔/毫升（范围为0.5 - 0.9微摩尔/毫升），而对照组的LPO平均水平为0.3±0.1微摩尔/毫升（范围为0.2 - 0.4微摩尔/毫升）。与健康对照者相比，HIV感染者的LPO平均水平显著更高（p值<0.0001）。HIV感染者的GSH平均水平为0.06±0.01微摩尔/毫升（范围为0.03 - 0.08）。健康对照者的GSH平均水平为0.09±0.01微摩尔/毫升（范围为0.05 - 0.1）。与健康对照者相比，HIV感染者的平均谷胱甘肽水平显著更低（p值<0.0001）。绝对CD4细胞与GSH水平之间存在显著正相关（rho = 0.182，p = 0.045）。HIV感染患者存在氧化应激增加的情况。补充抗氧化剂是否会减轻这种氧化应激仍不清楚。
