Department of Environmental Engineering Sciences, University of Florida, Gainesville, FL, USA.
J Appl Microbiol. 2010 Jan;108(1):315-24. doi: 10.1111/j.1365-2672.2009.04425.x.
To assess the impact of reaerosolization from liquid impingement methods on airborne virus sampling.
An AGI-30 impinger containing particles [MS2 bacteriophage or 30-nm polystyrene latex (PSL)] of known concentration was operated with sterile air. Reaerosolized particles as a function of sampling flow rate and particle concentration in the impinger collection liquid were characterized using a scanning mobility particle sizer. Reaerosolization from the impinger was also compared to that from a BioSampler. Results show that reaerosolization increases as flow rate increases. While the increased particle concentration in the impinger collection liquid leads to an increase in the reaerosolization of PSL particles, it does not necessarily lead to an increase in the reaerosolization of virus particles. Reaerosolization of virus particles begins to decrease as the particle concentration in the impinger collection liquid rises above 10(6) PFU ml(-1). This phenomenon results from aggregation of viral particles at high concentrations. Compared with micron-sized particles, nanosized virus particles are easier to aerosolize because of reduced inertia. Reaerosolization from the BioSampler is demonstrated to be significantly less than that from the impinger.
Reaerosolization from impingement sampling methods is a mode of loss in airborne virus sampling, although it is not as significant a limitation as the primary particle size of the aerosol. Utilizing a BioSampler coupled with short sampling periods to prevent high accumulative concentrations can minimize the impact of reaerosolization.
This study confirms reaerosolization of virus particles to be a mode of loss in impingement sampling and identifies methods to minimize the loss.
评估液体撞击法再气溶胶化对空气中病毒采样的影响。
采用含有已知浓度颗粒（MS2 噬菌体或 30nm 聚苯乙烯乳胶（PSL））的 AGI-30 撞击器，用无菌空气进行操作。使用扫描迁移率颗粒粒径仪对采样流速和撞击器收集液中颗粒浓度的再气溶胶化颗粒进行了特征描述。还将撞击器与生物采样器的再气溶胶化进行了比较。结果表明，再气溶胶化随流速的增加而增加。虽然撞击器收集液中颗粒浓度的增加导致 PSL 颗粒的再气溶胶化增加，但并不一定导致病毒颗粒的再气溶胶化增加。当撞击器收集液中的颗粒浓度超过 10(6)PFU ml(-1)时，病毒颗粒的再气溶胶化开始减少。这种现象是由于高浓度下病毒颗粒的聚集所致。与微米级颗粒相比，纳米级病毒颗粒由于惯性减小而更容易气溶胶化。与撞击器相比，生物采样器的再气溶胶化明显较少。
撞击采样方法的再气溶胶化是空气中病毒采样的一种损失模式，尽管它不像气溶胶的原始粒径那样是一个重要的限制因素。利用生物采样器并结合较短的采样时间来防止高累积浓度，可以最小化再气溶胶化的影响。
本研究证实了病毒颗粒的再气溶胶化为撞击采样的一种损失模式，并确定了最小化损失的方法。
