Department of Obstetrics and Gynecology, Graduate School of Medicine, Gifu University School of Medicine, 1-1 Yanagido, Gifu City 501-1194, Japan.
BMC Cancer. 2009 Dec 10;9:430. doi: 10.1186/1471-2407-9-430.
The inhibitor of DNA-binding (ID) has been involved in cell cycle regulation, apoptosis and angiogenesis. This prompted us to study ID functions in tumor advancement of ovarian cancers.
Sixty patients underwent surgery for ovarian cancers. In ovarian cancers, the levels of ID-1, ID-2 and ID-3 mRNAs were determined by real-time reverse transcription-polymerase chain reaction. The histoscore with the localization of ID-1 was determined by immunohistochemistry. Patient prognosis was analyzed with a 36-month survival rate. Microvessel counts were determined by immunohistochemistry for CD34 and factor VIII-related antigen.
ID-1 histoscores and mRNA levels both significantly (p < 0.001) increased in ovarian cancers according to clinical stage, regardless of histopathological type. Furthermore, 30 patients with high ID-1 expression had a lower survival rate (53%) compared to patients with low ID-1 expression (80%). ID-1 histoscores and mRNA levels significantly (p < 0.0001) correlated with microvessel counts in ovarian cancers.
ID-1 increased in ovarian cancer cells during tumor progression. Moreover, ID-1 expression levels correlated with microvessel counts. Therefore, ID-1 might work on tumor advancement via angiogenesis and is considered to be a candidate for a prognostic indicator in ovarian cancers.
DNA 结合抑制因子（ID）参与细胞周期调控、凋亡和血管生成。这促使我们研究 ID 在卵巢癌肿瘤进展中的作用。
60 名接受卵巢癌手术的患者。通过实时逆转录-聚合酶链反应确定卵巢癌中 ID-1、ID-2 和 ID-3 mRNA 的水平。通过免疫组织化学确定 ID-1 的定位组织评分。用 36 个月生存率分析患者预后。通过免疫组织化学用 CD34 和因子 VIII 相关抗原确定微血管计数。
根据临床分期，无论组织病理学类型如何，ID-1 组织评分和 mRNA 水平在卵巢癌中均显著（p < 0.001）升高。此外，30 名高 ID-1 表达的患者的生存率（53%）低于低 ID-1 表达的患者（80%）。ID-1 组织评分和 mRNA 水平与卵巢癌中的微血管计数显著相关（p < 0.0001）。
ID-1 在卵巢癌细胞中随着肿瘤的进展而增加。此外，ID-1 表达水平与微血管计数相关。因此，ID-1 可能通过血管生成作用于肿瘤进展，被认为是卵巢癌预后指标的候选者。
