Canadian Centre for DNA Barcoding, Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
Front Zool. 2009 Dec 10;6:30. doi: 10.1186/1742-9994-6-30.
This study reports progress in assembling a DNA barcode reference library for Ephemeroptera, Plecoptera, and Trichoptera ("EPTs") from a Canadian subarctic site, which is the focus of a comprehensive biodiversity inventory using DNA barcoding. These three groups of aquatic insects exhibit a moderate level of species diversity, making them ideal for testing the feasibility of DNA barcoding for routine biotic surveys. We explore the correlation between the morphological species delineations, DNA barcode-based haplotype clusters delimited by a sequence threshold (2%), and a threshold-free approach to biodiversity quantification--phylogenetic diversity.
A DNA barcode reference library is built for 112 EPT species for the focal region, consisting of 2277 COI sequences. Close correspondence was found between EPT morphospecies and haplotype clusters as designated using a standard threshold value. Similarly, the shapes of taxon accumulation curves based upon haplotype clusters were very similar to those generated using phylogenetic diversity accumulation curves, but were much more computationally efficient.
The results of this study will facilitate other lines of research on northern EPTs and also bode well for rapidly conducting initial biodiversity assessments in unknown EPT faunas.
本研究报告了在加拿大亚北极地区组装蜉蝣目、襀翅目和毛翅目（“EPTs”）DNA 条码参考文库的进展，该地区是使用 DNA 条码进行全面生物多样性调查的重点。这三个水生昆虫群体现出中等水平的物种多样性，非常适合测试 DNA 条码用于常规生物调查的可行性。我们探索了形态物种划分、通过序列阈值（2%）界定的 DNA 条码基于单倍型聚类以及无阈值生物多样性量化方法——系统发育多样性之间的相关性。
为焦点区域的 112 种 EPT 物种构建了一个 DNA 条码参考文库，其中包含 2277 个 COI 序列。使用标准阈值指定的 EPT 形态物种与单倍型聚类之间存在密切对应关系。同样，基于单倍型聚类生成的分类群积累曲线的形状与使用系统发育多样性积累曲线生成的形状非常相似，但计算效率要高得多。
本研究的结果将有助于北方 EPTs 的其他研究，并为快速进行未知 EPT 动物群的初始生物多样性评估提供良好的基础。
