Rabilloud T, Pennetier J L, Hibner U, Vincens P, Tarroux P, Rougeon F
Département d'Immunologie, Institut Pasteur, Paris, France.
Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1830-4. doi: 10.1073/pnas.88.5.1830.
Total nuclear proteins extracted from cell lines representing various stages of differentiation of mouse B lymphocytes were studied by computer analysis of two-dimensional gels. Of the 1438 spots present on the gels, 55 varied significantly in intensity during differentiation. The variations occurred most often in steps correlating with those classically defined for B-cell differentiation. Seventeen spots were not detectable in at least one of the stages (qualitative variations) and could represent switching on or off of genes coding for nuclear proteins. Detailed analysis of the 55 variable spots showed that they fall into small sets characterized by similar expression profiles, which argues for a combinatorial, multistep control mechanism of gene expression. In addition, analysis of the expression of all the nuclear proteins resolved on the gels clearly differentiated B-lineage cells from myeloid cells and suggested that the most important transition in B-cell differentiation occurs between the resting B cell and plasmocyte stages.
通过对二维凝胶的计算机分析，研究了从小鼠B淋巴细胞不同分化阶段的细胞系中提取的总核蛋白。凝胶上出现的1438个斑点中，有55个在分化过程中强度有显著变化。这些变化最常发生在与经典定义的B细胞分化步骤相关的阶段。17个斑点在至少一个阶段中无法检测到（定性变化），可能代表编码核蛋白的基因的开启或关闭。对55个可变斑点的详细分析表明，它们分为具有相似表达谱的小类，这表明基因表达存在组合式、多步骤控制机制。此外，对凝胶上解析的所有核蛋白的表达分析清楚地区分了B系细胞和髓系细胞，并表明B细胞分化中最重要的转变发生在静止B细胞和浆细胞阶段之间。
