RealSeal 对人成骨样 MG63 细胞的细胞毒性。

Cytotoxicity of RealSeal on human osteoblast-like MG63 cells.

机构信息

West China School of Stomatology, Sichuan University, Sichuan, China.

出版信息

J Endod. 2010 Jan;36(1):40-4. doi: 10.1016/j.joen.2009.09.002.

DOI:10.1016/j.joen.2009.09.002

PMID:20003933

Abstract

INTRODUCTION

The purpose of this study was to compare the cytotoxicity of a new root canal filling material (RealSeal system) with epoxy resin-based root canal sealer (AH Plus) and gutta-percha by using human osteoblast-like MG63 cells.

METHODS

Samples of each test material were fabricated in sterile cylindrical Teflon tubes of 4.4 mm diameter and 2 mm height. Extraction of specimens was carried out after setting in cell culture medium by using the ratio of 1.25 cm(2)/mL according to ISO 10993-5. Cytotoxicity was assessed by direct incubation of different extracts with MG63 cells at different time intervals. The dimethylthiazol diphenyltetrazolium bromide assay and flow cytometry were used to test the proliferation of MG63 cells. The results were analyzed with a Tukey test (alpha = 0.05).

RESULTS

The gutta-percha was nontoxic to MG63 cells in vitro, whereas RealSeal core material showed initial cytotoxic effect on MG63 cells after 1 day of culture, which disappeared after 3 days of culture. The RealSeal sealer was significantly more cytotoxic than AH Plus (P < .05) within 3 days. Flow cytometry showed that cell proliferation in RealSeal sealer and AH Plus group was inhibited.

CONCLUSIONS

The RealSeal sealer was significantly more toxic to MG63 cells than the AH Plus within 3 days, whereas RealSeal core material was similar with gutta-percha. Further research is required to evaluate the biocompatibility of RealSeal system comprehensively.

摘要

简介

本研究旨在通过人成骨样 MG63 细胞比较一种新型根管封闭材料（RealSeal 系统）与环氧树脂根管封闭剂（AH Plus）和牙胶的细胞毒性。

方法

将每种测试材料的样品制备在无菌的圆柱形聚四氟乙烯管中，直径为 4.4mm，高度为 2mm。根据 ISO 10993-5，按照 1.25cm²/mL 的比例，在细胞培养基中凝固后，从样本中提取试件。通过不同提取物与 MG63 细胞在不同时间间隔的直接孵育，评估细胞毒性。采用二甲基噻唑二苯基四氮唑溴盐法和流式细胞术检测 MG63 细胞的增殖。采用 Tukey 检验（α=0.05）对结果进行分析。

结果

体外实验中，牙胶对 MG63 细胞无毒性，而 RealSeal 核心材料在培养 1 天后对 MG63 细胞表现出初始细胞毒性作用，培养 3 天后这种作用消失。在培养 3 天内，RealSeal 密封剂的细胞毒性明显高于 AH Plus（P<0.05）。流式细胞术显示，RealSeal 密封剂和 AH Plus 组的细胞增殖受到抑制。